Abstract

The purpose of the study was to evaluate the production of lignin-modifying enzyme extracts and delignified biomass from agro-industrial wastes using white rot fungi (Inonotus sp. Sp2, Stereum hirsutum Ru-104, Bjerkandera sp. BOS55, Pleurotus eryngii IJFM 169 and Phanerochaete chrysosporium BKM-F-1767). These were screened based on their adaptability and colonization ability on different substrates, as well as by the Laccase, Manganese peroxidase, and Lignin peroxidase enzymatic production. Native strains (Inonotus sp. Sp2 and S. hirsutum Ru-104) showed the highest growth kinetics under the solid-substrate fermentation conditions and the growth rate parameters of the kinetic logistic model for the different substrates were between 0.39–0.81 (1/d) and 0.42–0.83 (1/d), respectively; the determination coefficients were ≥0.99. Inonotus sp. Sp2 was subsequently cultured in static flasks to produce crude enzyme extracts, obtaining manganese peroxidase activity levels of 18.5 and 31.3 (U/g) when growing in corn cob husk and spent tea leaves, respectively. Besides, it was to establish that the best conditions for lignin-modifying enzymes production using corn cob husk are 70% of initial moisture and 2.12 mm of particle size; reaching after 30 incubation days a manganese peroxidase activity of 21 ± 6 (U/g) under these conditions; enzyme that showed a suitable thermostability.

摘要

该研究的目的是评估使用白腐真菌（Inonotus sp. Sp2、Stereum hirsutum Ru-104、Bjerkandera sp. BOS55 、杏鲍菇IJFM 169 和Phanerochaete chrysosporium BKM-F-1767)。这些是根据它们在不同底物上的适应性和定植能力以及漆酶、锰过氧化物酶和木质素过氧化物酶的酶促生产来筛选的。本地菌株（Inonotus sp. Sp2 和S. hirsutumRu-104）在固体底物发酵条件下表现出最高的生长动力学，不同底物的动力学逻辑模型的生长速率参数在0.39-0.81（1 / d）和0.42-0.83（1 / d）之间，分别; 决定系数≥0.99。桦褐孔菌sp。随后在静态烧瓶中培养 Sp2 以产生粗酶提取物，在玉米芯壳和废茶叶中生长时分别获得 18.5 和 31.3 (U/g) 的锰过氧化物酶活性水平。此外，确定了利用玉米芯壳生产木质素改性酶的最佳条件是初始水分为70%，粒径为2.12 mm；在这些条件下，经过 30 天的孵育后，锰过氧化物酶活性达到 21 ± 6 (U/g)；显示出合适的热稳定性的酶。