The chromatographic techniques play a crucial role in analyzing materials in pharmaceutical, biochemical, chemical, biomedical, and forensic sectors. High purity and quality reference standards are inevitable for the precise and accurate estimation of analytes from the sample matrices. Here, the influence of reference material on the chromatographic estimation of a highly volatile analyte, ethylene oxide (EO), was studied using three batches of certified reference materials (CRMs). The relative uncertainties in the reference standards prepared with these CRMs are estimated as 0.036, 0.044, and 0.072 pu, respectively. The studies revealed that more than 98 % of the standard preparation uncertainty (U_SP) comes from the CRM concentration uncertainty. It also shows that more than 95 % of the combined uncertainty (U_C) was contributed by U_SP. Thus, the study highlights the necessity of good-quality CRMs and precise preparation methods for the accurate chromatographic analyses.

色谱技术在制药、生化、化学、生物医学和法医领域的材料分析中发挥着至关重要的作用。高纯度和质量的参考标准对于从样品基质中精确和准确地估计分析物是不可避免的。在这里，使用三批有证参考材料 (CRM) 研究了参考材料对高挥发性分析物环氧乙烷 (EO) 的色谱估计的影响。使用这些 CRM 制备的参考标准的相对不确定度估计分别为 0.036、0.044 和 0.072 pu。研究表明，超过 98% 的标准品制备不确定性（U _SP) 来自 CRM 浓度不确定性。它还表明，超过 95% 的组合不确定性 (U _C ) 是由 U _SP贡献的。因此，该研究强调了高质量 CRM 和精确制备方法对准确色谱分析的必要性。