Vibriosis is one of the major diseases leading to massive fish mortality. Probiotics may provide a potential alternative method to protect fish from pathogens and to promote a balanced environment minimizing the use of antibiotics and chemotherapy. The aims of this study were to (i) isolate and purify marine spore-former strains from Sardine and shrimp intestine, (ii) screen for bacteria with potential probiotic properties, and (iii) carry out their in vitro safety assessment using a subtractive procedure. Among 108 spore-former strains, five strains exhibited a strong antibacterial activity against Vibriosis such as Vibrio harveyi and Vibrio anguillarum. These selected strains were unaffected by high-temperature and gastrointestinal conditions; produced amylase, protease, and lipase activities; and showed high percentages of auto-aggregation and co-aggregation with pathogens, as well as a strong adhesion to fish mucus. Partial 16S rDNA gene sequencing and MALDI-TOF MS revealed that isolates are Bacillus amyloliquefaciens or Bacillus subtilis. All of them were susceptible to antibiotics, while hydrolic enzymes and virulence factors were not detected for B. subtilis S17. In conclusion, based on their proprieties and their safety assessment, B. subtilis S17 could serve as a potential probiotic candidate for aquaculture.

弧菌病是导致鱼类大量死亡的主要疾病之一。益生菌可能提供一种潜在的替代方法来保护鱼类免受病原体的侵害，并促进平衡环境，最大限度地减少抗生素和化学疗法的使用。本研究的目的是 (i) 从沙丁鱼和虾肠中分离和纯化海洋产孢子菌株，(ii) 筛选具有潜在益生菌特性的细菌，以及 (iii)体外使用减法程序进行安全性评估。在 108 株产芽孢菌株中，有 5 株对弧菌病表现出很强的抗菌活性，如哈维弧菌和鳗弧菌. 这些选定的菌株不受高温和胃肠道条件的影响；产生淀粉酶、蛋白酶和脂肪酶活性；并显示出高百分比的自动聚集和与病原体的共聚集，以及对鱼粘液的强烈粘附。部分 16S rDNA 基因测序和 MALDI-TOF MS 显示分离株是解淀粉芽孢杆菌或者枯草芽孢杆菌. 他们都对抗生素敏感，而没有检测到水解酶和毒力因子枯草芽孢杆菌S17。总之，根据他们的适当性和安全评估，枯草芽孢杆菌S17 可以作为水产养殖的潜在益生菌候选者。