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Fabrication of Carboxymethylcellulose-Gelatin Dissolving Microneedle Patch for Pain-Free, Efficient, and Controlled Transdermal Delivery of Insulin
Journal of Pharmaceutical Innovation ( IF 2.6 ) Pub Date : 2022-08-12 , DOI: 10.1007/s12247-022-09670-w
Baishali A Jana, Riyaz Ali Osmani, Sanket Jaiswal, Rinti Banerjee, Veera Venkata Satyanarayana Reddy Karri, Ashish Wadhwani

Purpose

Dissolving microneedle patches are attracting attention as they are associated with improved patient compliance and safety.

Methods

This study fabricated dissolving microneedle (DMN) patches from modified sodium carboxymethylcellulose (CMC) and gelatin might be as an alternative to subcutaneous injection for the transdermal delivery of insulin.

Results

The prepared DMNs exhibited excellent mechanical strength to penetrate the skin and confocal microscopy showed gradual diffuse from the puncture sites to deeper tissue up to more than 750–800 µm. The in vitro study was performed by a dissolution apparatus and ex vivo by the Franz diffusion cell system mainly quantified by human insulin ELISA kit where the release study exhibited excellent results with constant and stable delivery up to 96 ± 2%. The in vivo study results confirmed an ideal drug release profile up to 95 ± 2% until 8 h in 24-h study and thus insulin-loaded CMC-gelatin formulation is suitable for drug encapsulation, having excellent dissolving factor and penetration depth and avoiding drug loss. A home applicator played an important role in improving the efficacy and stability and faster insulin release rate. The plasma insulin level and blood glucose level (BGL) were calculated and were maintained and a stable decrease in 3–4 h was observed compared to SC injection route with the same insulin dose.

Conclusion

These results suggest that the CMC-gelatin DMN patch loaded with insulin has great potential in diabetes treatment for both type 1 and 2 diabetic patients in a relatively painless, rapid, and convenient manner.

Graphical Abstract



中文翻译:

羧甲基纤维素-明胶溶解微针贴片的制造,用于无痛、高效和可控的胰岛素经皮给药

目的

溶解性微针贴片引起了人们的关注,因为它们与提高患者的依从性和安全性有关。

方法

本研究使用改性羧甲基纤维素钠 (CMC) 和明胶制造溶解微针 (DMN) 贴片,可作为皮下注射的替代品,用于经皮递送胰岛素。

结果

制备的 DMN 表现出优异的机械强度以穿透皮肤,共聚焦显微镜显示从穿刺部位逐渐扩散到更深的组织,深度超过 750-800 µm。体外研究通过溶出仪进行,Franz 扩散池系统进行体外研究,主要通过人胰岛素 ELISA 试剂盒进行量化,其中释放研究显示出优异的结果,持续稳定的递送高达 96 ± 2%。体内研究结果证实,在 24 小时研究中,直至 8 小时的理想药物释放曲线高达 95 ± 2%,因此载有胰岛素的 CMC 明胶制剂适用于药物包封,具有出色的溶解因子和渗透深度,避免药物失利。家用涂药器在提高疗效和稳定性以及更快的胰岛素释放速度方面发挥了重要作用。

结论

这些结果表明,载有胰岛素的 CMC-明胶 DMN 贴片在以相对无痛、快速和方便的方式治疗 1 型和 2 型糖尿病患者的糖尿病治疗中具有巨大潜力。

图形概要

更新日期:2022-08-13
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