Early rapid screening diagnostic assay is essential for the identification, prevention, and evaluation of many contagious or refractory diseases. The optical density transducer created by platinum nanoparticles (PtNPs) (OD-CRISPR) is reported in the present research as a cheap and easy-to-execute CRISPR/Cas12a-based diagnostic platform. The OD-CRISPR uses PtNPs, with ultra-high peroxidase-mimicking activity, to increase the detection sensitivity, thereby enabling the reduction of detection time and cost. The OD-CRISPR can be utilized to identify nucleic acid or protein biomarkers within an incubation time of 30–40min in clinical specimens. In the case of taking severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) N gene as an instance, when compared to a quantitative reverse transcription-polymerase chain reaction (RT-qPCR), the OD-CRISPR test attains a sensitivity of 79.17% and a specificity of 100%. In terms of detecting prostate-specific antigen (PSA), aptamer-based OD-CRISPR assay achieves the least discoverable concentration of 0.01 ng mL⁻¹. In general, the OD-CRISPR can detect nucleic acid and protein biomarkers, and is a potential strategy for early rapid screening diagnostic tools.

早期快速筛查诊断测定对于许多传染性或难治性疾病的识别、预防和评估至关重要。由铂纳米粒子 (PtNPs) (OD-CRISPR) 创建的光密度传感器在本研究中被报道为一种廉价且易于执行的基于 CRISPR/Cas12a 的诊断平台。OD-CRISPR 使用具有超高过氧化物酶模拟活性的 PtNPs 来提高检测灵敏度，从而减少检测时间和成本。OD-CRISPR 可用于在 30-40 分钟的孵育时间内识别临床标本中的核酸或蛋白质生物标志物。以严重急性呼吸综合征冠状病毒2（SARS-CoV-2）N基因为例，与定量逆转录聚合酶链反应（RT-qPCR）相比，OD-CRISPR 测试的灵敏度为 79.17%，特异性为 100%。在检测前列腺特异性抗原 (PSA) 方面，基于适配体的 OD-CRISPR 检测可达到 0.01 ng mL 的最低可检测浓度^-1。一般来说，OD-CRISPR可以检测核酸和蛋白质生物标志物，是早期快速筛查诊断工具的潜在策略。