SiO₂@TiO₂ thin film synthesis and their UV protective application in microorganisms and the molecular response in the embryonic development of zebrafish were elucidated. SiO₂@TiO₂ thin films were fabricated on glass substrates by the sol–gel dip-coating technique. The UV screening effects of SiO₂@TiO₂ thin films as a protective layer to enhance the growth of Pseudomonas sp. and Azospirillum sp. were studied. We also observed that the survival rate and development of embryos of zebrafish increase with the increase in film thickness after UV exposure compared to the control. UV-B induced damage was observed in the development of eyes, cartilages, bones and fins of zebrafish. To elucidate the molecular mechanism involved, the expression of the pitx3 and sparc (osteonectin) genes was studied in UV-B exposed and SiO₂@TiO₂ thin film protected groups. The results indicated that pitx3 expression decreased and sparc expression increased under UV-B exposure. The expression level of the pitx3 and sparc genes was studied in the SiO₂@TiO₂ thin film protected group and compared to the control to determine the UV-B protective effect of SiO₂@TiO₂ thin films in the embryonic development of zebrafish. In conclusion, SiO₂@TiO₂ thin films act as a protective screen against UV-B radiation which may be useful for biomedical and agricultural applications.

中文翻译：

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SiO2掺杂TiO2薄膜的UV-B保护作用调节斑马鱼胚胎发育过程中pitx3和sparc的表达

阐明了SiO ₂ @TiO ₂薄膜的合成及其在微生物中的紫外线防护应用以及斑马鱼胚胎发育中的分子反应。SiO ₂ @TiO ₂薄膜通过溶胶-凝胶浸涂技术在玻璃基板上制备。_{SiO 2} @TiO ₂薄膜作为保护层促进假单胞菌生长的紫外线屏蔽作用。和固氮螺菌属sp。进行了研究。我们还观察到，与对照相比，斑马鱼胚胎的存活率和发育随着紫外线照射后薄膜厚度的增加而增加。在斑马鱼的眼睛、软骨、骨骼和鳍的发育中观察到 UV-B 引起的损伤。为了阐明所涉及的分子机制，在 UV-B 暴露和 SiO ₂ @TiO ₂薄膜保护基团中研究了 pitx3 和 sparc（骨连接素）基因的表达。结果表明，在UV-B照射下pitx3表达降低，sparc表达增加。_{在SiO 2} @TiO ₂中研究pitx3和sparc基因的表达水平薄膜保护组并与对照进行比较，以确定SiO ₂ @TiO ₂薄膜在斑马鱼胚胎发育中的UV-B保护作用。总之，SiO ₂ @TiO ₂薄膜可作为 UV-B 辐射的保护屏，可用于生物医学和农业应用。