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SARS-CoV-2 and other respiratory pathogens are detected in continuous air samples from congregate settings
Nature Communications ( IF 16.6 ) Pub Date : 2022-08-11 , DOI: 10.1038/s41467-022-32406-w
Mitchell D Ramuta 1 , Christina M Newman 1 , Savannah F Brakefield 1 , Miranda R Stauss 2 , Roger W Wiseman 1, 2 , Amanda Kita-Yarbro 3 , Eli J O'Connor 4 , Neeti Dahal 5 , Ailam Lim 5 , Keith P Poulsen 5 , Nasia Safdar 6 , John A Marx 6 , Molly A Accola 6 , William M Rehrauer 1, 6 , Julia A Zimmer 7 , Manjeet Khubbar 7 , Lucas J Beversdorf 7 , Emma C Boehm 8 , David Castañeda 8 , Clayton Rushford 9 , Devon A Gregory 9 , Joseph D Yao 10 , Sanjib Bhattacharyya 7 , Marc C Johnson 9 , Matthew T Aliota 8 , Thomas C Friedrich 11 , David H O'Connor 1, 2 , Shelby L O'Connor 1, 2
Affiliation  

Two years after the emergence of SARS-CoV-2, there is still a need for better ways to assess the risk of transmission in congregate spaces. We deployed active air samplers to monitor the presence of SARS-CoV-2 in real-world settings across communities in the Upper Midwestern states of Wisconsin and Minnesota. Over 29 weeks, we collected 527 air samples from 15 congregate settings. We detected 106 samples that were positive for SARS-CoV-2 viral RNA, demonstrating that SARS-CoV-2 can be detected in continuous air samples collected from a variety of real-world settings. We expanded the utility of air surveillance to test for 40 other respiratory pathogens. Surveillance data revealed differences in timing and location of SARS-CoV-2 and influenza A virus detection. In addition, we obtained SARS-CoV-2 genome sequences from air samples to identify variant lineages. Collectively, this shows air sampling is a scalable, high throughput surveillance tool that could be used in conjunction with other methods for detecting respiratory pathogens in congregate settings.



中文翻译:

在聚集场所的连续空气样本中检测到 SARS-CoV-2 和其他呼吸道病原体

SARS-CoV-2 出现两年后,仍然需要更好的方法来评估聚集空间中的传播风险。我们部署了主动空气采样器来监测威斯康星州和明尼苏达州中西部上西部各社区现实环境中 SARS-CoV-2 的存在情况。在 29 周的时间里,我们从 15 个聚集场所收集了 527 个空气样本。我们检测到 106 个样本的 SARS-CoV-2 病毒 RNA 呈阳性,这表明可以在从各种现实环境中收集的连续空气样本中检测到 SARS-CoV-2。我们扩大了空气监测的用途,以检测 40 种其他呼吸道病原体。监测数据显示 SARS-CoV-2 和甲型流感病毒检测的时间和地点存在差异。此外,我们从空气样本中获得了 SARS-CoV-2 基因组序列,以鉴定变异谱系。总的来说,这表明空气采样是一种可扩展的、高通量的监测工具,可以与其他方法结合使用,在聚集环境中检测呼吸道病原体。

更新日期:2022-08-11
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