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Immunogenic Evaluation of MPEG-PCL & PLGA Nanoparticles Containing Klebsiella pneumoniae K2O1 Capsular Antigen in Pulmonary Infection Model of Mice
IEEE Transactions on NanoBioscience ( IF 3.9 ) Pub Date : 2022-08-01 , DOI: 10.1109/tnb.2022.3195483
Parivash Ghaderinia 1 , Reza Shapouri 2 , Kobra Rostamizadeh 3 , Alireza Khodavandi 4 , Mehdi Mahdavi 5
Affiliation  

Klebsiella pneumoniae can cause destructive changes to human lungs if aspirated. The present study aimed to evaluate the immunogenicity of the carriers of Poly lactic-co-glycolic acid (PLGA) and Methoxypoly(ethylene glycol) Poly(caprolactone) (MPEG-PCL) nanoparticles containing the capsular antigen of Klebsiella pneumoniae K2O1 in a model of pulmonary infection in mice as a vaccine candidate. Capsule antigen was extracted from K.pneumoniae K2O1 strain 1053 ATCC 10031 and transported with PLGA or MPEG-PCL nanoparticles as a vaccine in an animal model. The results of FT-IR and AFM confirmed the presence of antigen functional groups in the nanoparticle structure, and semi-spherical shape of the nanoparticles, respectively. The capsular polysaccharide was also used to evaluate the febrileness of the designed vaccine candidates based on the rabbits’ pattern, and mortality due to the vaccine candidates in the mice. No fever was observed, and no mortality was observed in the mice. According to the results, the vaccine candidates designed to control the cause of pulmonary infections were effective in the liver, spleen, and lungs of the animals with the ability to enter the first stage of the clinical trial phase.

中文翻译:

含肺炎克雷伯菌 K2O1 荚膜抗原的 MPEG-PCL & PLGA 纳米颗粒在小鼠肺部感染模型中的免疫原性评价

肺炎克雷伯菌如果被吸入,会对人的肺部造成破坏性变化。本研究旨在评估含有肺炎克雷伯菌 K2O1 荚膜抗原的聚乳酸-乙醇酸共聚物 (PLGA) 和甲氧基聚乙二醇聚己内酯 (MPEG-PCL) 纳米颗粒载体的免疫原性。小鼠肺部感染作为候选疫苗。从 K.pneumoniae K2O1 菌株 1053 ATCC 10031 中提取荚膜抗原,并在动物模型中作为疫苗与 PLGA 或 MPEG-PCL 纳米颗粒一起运输。FT-IR 和 AFM 的结果分别证实了纳米颗粒结构中存在抗原官能团,纳米颗粒呈半球形。荚膜多糖还用于根据兔子的模式评估设计的候选疫苗的发热性,以及小鼠因候选疫苗引起的死亡率。没有观察到发烧,并且在小鼠中没有观察到死亡。根据结果​​,设计用于控制肺部感染病因的候选疫苗对动物的肝脏、脾脏和肺部有效,有能力进入临床试验阶段的第一阶段。
更新日期:2022-08-01
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