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NAC transcription factor TgNAP promotes tulip petal senescence.
Plant Physiology ( IF 7.4 ) Pub Date : 2022-10-27 , DOI: 10.1093/plphys/kiac351
Lin Meng 1, 2 , Haipo Yang 1, 2 , Lin Xiang 1 , Yanping Wang 1, 2 , Zhulong Chan 1
Affiliation  

Petal senescence is a crucial determinant for ornamental quality and economic value of floral crops. Salicylic acid (SA) and reactive oxygen species (ROS) are two prominent factors involved in plant senescence regulation. In this study, tulip TgNAP (NAC-like, activated by APETALA3/PISTILLATA) was characterized as positively regulating tulip petal senescence through dually regulating SA biosynthesis and ROS detoxification pathways. TgNAP was upregulated in senescing petals of tulip while exogenous SA and H2O2 treatments substantially promoted petal senescence in tulip. Silencing of TgNAP by VIGS assay delayed SA and H2O2-induced petal senescence in tulip, whereas overexpression of TgNAP promoted the senescence process in Arabidopsis (Arabidopsis thaliana) plants. Additionally, inhibition of SA biosynthesis prolonged the lifespan of TgNAP-silenced petal discs. Further evidence indicated that TgNAP activates the transcriptions of two key SA biosynthetic genes ISOCHORISMATE SYNTHASE 1 (TgICS1) and PHENYLALANINE AMMONIA-LYASE 1 (TgPAL1) through directly binding to their promoter regions. Meanwhile, TgNAP repressed ROS scavenging by directly inhibiting PEROXIDASE 12 (POD12) and POD17 expression. Taken together, these results indicate that TgNAP enhances SA biosynthesis and ROS accumulation to positively regulate petal senescence in tulip.

中文翻译:

NAC转录因子TgNAP促进郁金香花瓣衰老。

花瓣衰老是花卉作物观赏质量和经济价值的关键决定因素。水杨酸(SA)和活性氧(ROS)是参与植物衰老调节的两个重要因素。在本研究中,郁金香 TgNAP(NAC 样,由 APETALA3/PISTILLATA 激活)的特点是通过双重调节 SA 生物合成和 ROS 解毒途径,积极调节郁金香花瓣衰老。TgNAP 在郁金香衰老花瓣中上调,而外源 SA 和 H2O2 处理显着促进郁金香花瓣衰老。VIGS 测定中 TgNAP 的沉默延迟了 SA 和 H2O2 诱导的郁金香花瓣衰老,而 TgNAP 的过表达促进了拟南芥 (Arabidopsis thaliana) 植物的衰老过程。此外,抑制 SA 生物合成可延长 TgNAP 沉默花瓣盘的寿命。进一步的证据表明,TgNAP 通过直接结合至两个关键的 SA 生物合成基因 ISOCHORISMATE SYNTHASE 1 (TgICS1) 和 PHENYLALANINE AMMONIA-LYASE 1 (TgPAL1) 的启动子区域来激活它们的转录。同时,TgNAP 通过直接抑制过氧化物酶 12 (POD12) 和 POD17 表达来抑制 ROS 清除。综上所述,这些结果表明 TgNAP 增强 SA 生物合成和 ROS 积累,从而积极调节郁金香花瓣衰老。
更新日期:2022-07-28
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