Chronic wounds have become an important factor hindering human health, affecting tens of millions of people worldwide, especially diabetic wounds. Based on the antibacterial properties of chitosan, the angiogenesis promoting effect of vaccarin (VAC) and the anti-inflammatory effect of hypaphorine (HYP), nanoparticles with high bioavailability were prepared. VAC, HYP and chitosan nanoparticles (VAC + HYP-NPS) were used to the treatment of chronic wounds. Transmission electron microscopy (TEM) images showed the nanoparticles were spherical. ZetaPALS showed the potential of nanoparticles were −12.8 ± 5.53 mV and the size were 166.8 ± 29.95 nm. Methyl thiazolyl tetrazolium (MTT) assay showed that VAC + HYP-NPS had no toxicity and the biocompatibility was satisfactory. In the treatment of chronic wounds in diabetic rats, VAC + HYP-NPS significantly promoted the re-epithelialization of chronic wounds and accelerated the healing of chronic wounds. In the process of chronic wounds healing, VAC + HYP-NPS played the antibacterial effect of chitosan, the angiogenic effect of VAC and the anti-inflammatory effect of HYP, and finally promoted the chronic wounds healing. Overall, the developed VAC + HYP-NPS have potential application in chronic wounds healing. In view of the complexity of the causes of chronic wounds, multi-target drug administration may be an effective way to treat chronic wounds.

慢性伤口已成为阻碍人类健康的重要因素，影响着全球数以千万计的人，尤其是糖尿病伤口。基于壳聚糖的抗菌特性、痘苗碱（VAC）的血管生成促进作用和hypaphorine（HYP）的抗炎作用，制备了具有高生物利用度的纳米颗粒。VAC、HYP和壳聚糖纳米粒子（VAC + HYP-NPS）用于治疗慢性伤口。透射电子显微镜 (TEM) 图像显示纳米颗粒是球形的。ZetaPALS 显示纳米粒子的电位为 -12.8 ± 5.53 mV，尺寸为 166.8 ± 29.95 nm。甲基噻唑基四唑（MTT）测定表明VAC+HYP-NPS无毒，生物相容性良好。在糖尿病大鼠慢性伤口的治疗中，VAC+HYP-NPS显着促进慢性创面再上皮化，加速慢性创面愈合。在慢性创面愈合过程中，VAC+HYP-NPS发挥壳聚糖的抗菌作用、VAC的血管生成作用和HYP的抗炎作用，最终促进慢性创面的愈合。总体而言，开发的 VAC + HYP-NPS 在慢性伤口愈合中具有潜在应用。鉴于慢性创面病因的复杂性，多靶点给药可能是治疗慢性创面的有效方法。最终促进慢性伤口愈合。总体而言，开发的 VAC + HYP-NPS 在慢性伤口愈合中具有潜在应用。鉴于慢性创面病因的复杂性，多靶点给药可能是治疗慢性创面的有效方法。最终促进慢性伤口愈合。总体而言，开发的 VAC + HYP-NPS 在慢性伤口愈合中具有潜在应用。鉴于慢性创面病因的复杂性，多靶点给药可能是治疗慢性创面的有效方法。