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Transcription factors ZmNF-YA1 and ZmNF-YB16 regulate plant growth and drought tolerance in maize.
Plant Physiology ( IF 7.4 ) Pub Date : 2022-09-28 , DOI: 10.1093/plphys/kiac340
Yaling Yang 1 , Baomei Wang 1 , Jiemin Wang 1 , Chunmei He 2 , Dengfeng Zhang 3 , Peng Li 1 , Juren Zhang 1 , Zhaoxia Li 1
Affiliation  

The identification of drought stress regulatory genes is crucial for the genetic improvement of maize (Zea mays L.) yield. Nuclear factors Y (NF-Ys) are important transcription factors, but their roles in the drought stress tolerance of plants and underlying molecular mechanisms are largely unknown. In this work, we used yeast two-hybrid screening to identify potential interactors of ZmNF-YB16 and confirmed the interaction between ZmNF-YA1 and ZmNF-YB16-YC17 and between ZmNF-YA7 and ZmNF-YB16-YC17. ZmNF-YB16 interacted with ZmNF-YC17 via its histone fold domain to form a heterodimer in the cytoplasm and then entered the nucleus to form a heterotrimer with ZmNF-YA1 or ZmNF-YA7 under osmotic stress. Overexpression of ZmNF-YA1 improved drought and salt stress tolerance and root development of maize, whereas zmnf-ya1 mutants exhibited drought and salt stress sensitivity. ZmNF-YA1-mediated transcriptional regulation, especially in JA signaling, histone modification, and chromatin remodeling, could underlie the altered stress tolerance of zmnf-ya1 mutant plants. ZmNF-YA1 bound to promoter CCAAT motifs and directly regulated the expression of multiple genes that play important roles in stress responses and plant development. Comparison of ZmNF-YB16- and ZmNF-YA1-regulated genes showed that ZmNF-YA1 and ZmNF-YB16 have similar biological functions in stress responses but varied functions in other biological processes. Taken together, ZmNF-YA1 is a positive regulator of plant drought and salt stress responses and is involved in the root development of maize, and ZmNF-Y complexes with different subunits may have discrepant functions.

中文翻译:

转录因子 ZmNF-YA1 和 ZmNF-YB16 调节玉米的植物生长和耐旱性。

干旱胁迫调控基因的鉴定对于玉米(Zea mays L.)产量的遗传改良至关重要。核因子 Y (NF-Ys) 是重要的转录因子,但它们在植物耐旱胁迫中的作用和潜在的分子机制在很大程度上是未知的。在这项工作中,我们使用酵母双杂交筛选来鉴定 ZmNF-YB16 的潜在相互作用物,并确认 ZmNF-YA1 和 ZmNF-YB16-YC17 之间以及 ZmNF-YA7 和 ZmNF-YB16-YC17 之间的相互作用。ZmNF-YB16通过其组蛋白折叠结构域与ZmNF-YC17相互作用,在细胞质中形成异二聚体,然后在渗透胁迫下进入细胞核与ZmNF-YA1或ZmNF-YA7形成异源三聚体。ZmNF-YA1的过表达改善了玉米的干旱和盐胁迫耐受性和根系发育,而 zmnf-ya1 突变体表现出干旱和盐胁迫敏感性。ZmNF-YA1 介导的转录调控,特别是在 JA 信号传导、组蛋白修饰和染色质重塑中,可能是 zmnf-ya1 突变植物改变胁迫耐受性的基础。ZmNF-YA1 与启动子 CCAAT 基序结合并直接调节在胁迫响应和植物发育中起重要作用的多个基因的表达。ZmNF-YB16 和 ZmNF-YA1 调节基因的比较表明,ZmNF-YA1 和 ZmNF-YB16 在应激反应中具有相似的生物学功能,但在其他生物学过程中具有不同的功能。综上所述,ZmNF-YA1 是植物干旱和盐胁迫响应的正调节因子,参与玉米的根系发育,不同亚基的 ZmNF-Y 复合物可能具有不同的功能。
更新日期:2022-07-21
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