Chilo suppressalis is one of the most prevalent and damaging rice pests, causing significant economic losses each year. Chemical control is currently the primary method of controlling C. suppressalis. However, the indiscriminate use of chemical insecticides increases pest resistance, pollutes the environment and poses a significant health threat to humans and livestock, highlighting the need to find safer, more pest-specific and more effective alternatives to pest control. Plant-mediated RNA interference (RNAi) is a promising agricultural pest control method that is highly pest-specific and has less of an impact on the environment. Using multi-sgRNAs/Cas9 technology to delete Fatty acyl-CoA reductase (FAR) of C. suppressalis in the G0 generation, we show that downregulating FAR transcription may significantly increase the mortality rate and darken the epidermis of C. suppressalis compared with the control. Subsequently, we developed dsFAR transgenic rice lines using Agrobacterium-mediated genetic transformation and then screened three strains expressing dsFAR at high levels using transcriptional level analysis. Using transgenic rice stems, a laboratory feeding bioassay indicated that at least one line (L#10) displayed a particularly high level of insect resistance, with an insect mortality rate of more than 80%. In the field trials, dsFAR transgenic rice displayed high levels of resistance to C. suppressalis damage. Collectively, these results suggest the potential of a new environment-friendly, species-specific strategy for rice pest management.

中文翻译：

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FAR基因敲除显着抑制二化螟存活，双链FAR基因在水稻中的表达表现出很强的抗虫性

二化螟是最普遍和最具破坏性的水稻害虫之一，每年造成重大经济损失。化学防治是目前防治二化螟的主要方法。然而，滥用化学杀虫剂会增加害虫的抗药性，污染环境并对人类和牲畜的健康构成重大威胁，这凸显了寻找更安全、更针对害虫和更有效的害虫控制替代品的必要性。植物介导的 RNA 干扰 (RNAi) 是一种很有前途的农业害虫防治方法，它具有高度的害虫特异性并且对环境的影响较小。利用multi-sgRNAs/Cas9技术去除二化螟的脂酰辅酶A还原酶（FAR）在 G0 代中，我们发现与对照相比，下调FAR转录可能会显着增加二化螟的死亡率并使表皮变暗。随后，我们使用农杆菌介导的遗传转化开发了 dsFAR 转基因水稻品系，然后使用转录水平分析筛选了三个高水平表达 dsFAR 的菌株。使用转基因水稻茎，一项实验室饲喂生物测定表明，至少一个品系（L#10）表现出特别高水平的抗虫性，昆虫死亡率超过 80%。在田间试验中， dsFAR转基因水稻对二化螟表现出高水平的抗性损害。总的来说，这些结果表明了一种新的环境友好的、针对特定物种的水稻害虫管理策略的潜力。