We introduce a novel electrochemical protein quantitation based on the shotgun biotin tagging of proteins prior to their interfacial immunocapture and polymeric enzyme tagging. The highly amplified faradaic signals generated from a novel ferrocene-tyramine adduct enable fg mL⁻¹ (attomolar) levels of detection and span cross a 5 orders of magnitude dynamic range. This work supports ultrasensitive protein marker detection in a single antibody immunoassay format.

中文翻译：

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在超灵敏电化学免疫分析中催化放大法拉第鸟枪标记

我们介绍了一种新的电化学蛋白质定量方法，该方法基于蛋白质的鸟枪生物素标记，然后再进行界面免疫捕获和聚合酶标记。由新型二茂铁-酪胺加合物产生的高度放大的法拉第信号可实现 fg mL ^-1（阿摩尔）水平的检测，并跨越 5 个数量级的动态范围。这项工作支持以单一抗体免疫测定形式进行超灵敏蛋白质标记检测。