Comprehending the effects of synthetic nanomaterials on natural microorganisms is critical for the development of emerging nanotechnologies. Compared to artificial inactivation of microbes, the up-regulation of biological functions should be more attractive due to the possibility of discovering unexpected properties. Herein, a nanoengineering strategy was employed to tailor g-C₃N₄ for the metabolic regulation of algae. We found that surface protonated g-C₃N₄ (P-C₃N₄) as a nanopolymeric elicitor enabled the reinforced biological activity of Microcystis aeruginosa and Scenedesmus for harmful substances removal. Metabolomics analysis suggested that synthetic nanoarchitectures induced moderate oxidative stress of algae, with up-regulated biosynthesis of extracellular polymeric substances (EPS) for resisting the physiological damage caused by toxic substances in water. The formation of oxidative ^.O₂^– contributed to over five-fold enhancement in the biodecomposition of harmful aniline. Our study demonstrates a synergistic biotic-abiotic platform with valuable outcomes for various customized applications.

了解合成纳米材料对天然微生物的影响对于新兴纳米技术的发展至关重要。与微生物的人工灭活相比，由于可能发现意想不到的特性，生物功能的上调应该更具吸引力。在此，采用纳米工程策略来定制gC ₃ N ₄用于藻类的代谢调节。我们发现表面质子化的 gC ₃ N ₄ (PC ₃ N ₄ ) 作为纳米聚合物引发剂能够增强铜绿微囊藻和栅藻的生物活性用于去除有害物质。代谢组学分析表明，合成纳米结构可诱导藻类的中度氧化应激，上调细胞外聚合物（EPS）的生物合成，以抵抗水中有毒物质造成的生理损伤。形成氧化性^。O ₂ ^–有助于将有害苯胺的生物分解提高五倍以上。我们的研究展示了一个协同的生物-非生物平台，可为各种定制应用提供有价值的成果。