当前位置: X-MOL 学术Nat. Commun. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Thy1 marks a distinct population of slow-cycling stem cells in the mouse epidermis
Nature Communications ( IF 16.6 ) Pub Date : 2022-08-08 , DOI: 10.1038/s41467-022-31629-1
Elle Koren 1, 2, 3 , Alona Feldman 1, 2 , Marianna Yusupova 1, 2 , Avihay Kadosh 4 , Egor Sedov 1, 2 , Roi Ankawa 1, 2 , Yahav Yosefzon 1, 2 , Waseem Nasser 5 , Stefanie Gerstberger 6 , Liam B Kimel 4 , Noa Priselac 1, 2 , Samara Brown 7 , Sam Sharma 7 , Travis Gorenc 7 , Ruby Shalom-Feuerstein 5 , Hermann Steller 7 , Tom Shemesh 4 , Yaron Fuchs 1, 2
Affiliation  

The presence of distinct stem cells that maintain the interfollicular epidermis is highly debated. Here, we report a population of keratinocytes, marked by Thy1, in the basal layer of the interfollicular epidermis. We find that epidermal cells expressing differential levels of Thy1 display distinct transcriptional signatures. Thy1+ keratinocytes do not express T cell markers, express a unique transcriptional profile, cycle significantly slower than basal epidermal progenitors and display significant expansion potential in vitro. Multicolor lineage tracing analyses and mathematical modeling reveal that Thy1+ basal keratinocytes do not compete neutrally alike interfollicular progenitors and contribute long-term to both epidermal replenishment and wound repair. Importantly, ablation of Thy1+ cells strongly impairs these processes, thus indicating the non-redundant function of Thy1+ stem cells in the epidermis. Collectively, these results reveal a distinct stem cell population that plays a critical role in epidermal homeostasis and repair.



中文翻译:

Thy1 标记了小鼠表皮中独特的慢周期干细胞群

维持滤泡间表皮的不同干细胞的存在存在争议。在这里,我们报告了毛囊间表皮基底层中以 Thy1 为标志的角质形成细胞群。我们发现表达不同水平 Thy1 的表皮细胞表现出不同的转录特征。Thy1 +角质形成细胞不表达 T 细胞标记,表达独特的转录谱,周期明显慢于基底表皮祖细胞,并在体外表现出显着的扩增潜力。多色谱系追踪分析和数学模型表明,Thy1 +基底角质形成细胞不会像毛囊间祖细胞一样进行中性竞争,并且对表皮补充和伤口修复有长期贡献。重要的是,Thy1 +细胞的消融会强烈损害这些过程,从而表明 Thy1 +干细胞在表皮中的非冗余功能。总的来说,这些结果揭示了一种独特的干细胞群,在表皮稳态和修复中发挥着关键作用。

更新日期:2022-08-08
down
wechat
bug