Heart failure (HF) is a chronic cardiovascular disease that represents main cause of mortality worldwide, particularly for elderly. N-terminal pro-brain natriuretic peptide (NT-proBNP) was identified as the gold standard biomarker for HF diagnosis and therapy monitoring. Presently, saliva analysis represents an emerging and powerful tool for clinical applications and electrochemical immunosensors have shown their potential in Healthcare applications as selective and reliable systems for detecting clinical biomarkers. This work presents the detection of NT-proBNP in saliva samples by an immunologically modified Field effect Transistor (IMFET). TESUD ((11-triethoxysilyl) undecanal) was used as cross-linker to immobilise anti-NT-proBNP antibody onto the device. Our IMFET that was then tested in different matrices (e.g. phosphate buffered saline (PBS), artificial saliva and human saliva) using electrochemical impedance spectroscopy (EIS), and it resulted selective to NT-proBNP with good sensitivity (detection limit of 0.02 pg/mL) and a wide linear range (0.02–1 pg/mL and 0.5–20 pg/mL). Finally, NT-proBNP concentration in ten saliva samples was determined by performing the standard addition method. An enzyme-linked immunosorbent assay was used for confirming IMFET results, highlighting both IMFET accuracy (analyte recovery of 99 ± 8%) and precision (coefficient of variation always <10%), and supporting the suitability of the device for determining salivary NT-proBNP.

心力衰竭 (HF) 是一种慢性心血管疾病，是全球死亡的主要原因，尤其是老年人。N端脑利钠肽前体（NT-proBNP）被确定为HF诊断和治疗监测的金标准生物标志物。目前，唾液分析代表了一种新兴且强大的临床应用工具，电化学免疫传感器已在医疗保健应用中显示出其作为检测临床生物标志物的选择性和可靠系统的潜力。这项工作介绍了通过免疫修饰的场效应晶体管 (IMFET) 在唾液样本中检测 NT-proBNP。TESUD（（11-三乙氧基甲硅烷基）十一醛）用作交联剂以将抗 NT-proBNP 抗体固定在装置上。我们的 IMFET 然后在不同的基质中进行了测试（例如磷酸盐缓冲盐水 (PBS)，使用电化学阻抗谱 (EIS) 对人工唾液和人唾液进行检测，对 NT-proBNP 具有选择性，具有良好的灵敏度（检测限为 0.02 pg/mL）和较宽的线性范围（0.02-1 pg/mL 和 0.5-20皮克/毫升）。最后，通过标准添加法测定十个唾液样品中的 NT-proBNP 浓度。酶联免疫吸附测定用于确认 IMFET 结果，突出了 IMFET 准确度（分析物回收率为 99 ± 8%）和精密度（变异系数始终 <10%），并支持该设备用于确定唾液 NT- proBNP。5–20 皮克/毫升）。最后，通过标准添加法测定十个唾液样品中的 NT-proBNP 浓度。酶联免疫吸附测定用于确认 IMFET 结果，突出了 IMFET 准确度（分析物回收率为 99 ± 8%）和精密度（变异系数始终 <10%），并支持该设备用于确定唾液 NT- proBNP。5–20 皮克/毫升）。最后，通过标准添加法测定十个唾液样品中的 NT-proBNP 浓度。酶联免疫吸附测定用于确认 IMFET 结果，突出了 IMFET 准确度（分析物回收率为 99 ± 8%）和精密度（变异系数始终 <10%），并支持该设备用于确定唾液 NT- proBNP。