In electrochemiluminescence(ECL) field, the shortage of potential-resolved luminophores severiously limited the development of biosensors for accurate assay such as ratiometric biosensors. In this work, N,N′-dimethyl-3,4,9,10-perylenedicarboximide(PDI-CH₃) and graphite-like carbon nitride(g-C₃N₄) nanosheet was firstly reported to produce potential-resolved ECL with coreactant K₂S₂O₈ between 0 and − 1.2 V. And there was good overlap between the ECL spectrum of g-C₃N₄ and the absorption spectra of PDI-CH₃. Moreover, PDI-CH₃ and g-C₃N₄ were used to construct ECL ratiometric system for sandwiched immunosensors to detect tumor marker carcinoembryonic antigen (CEA), in which g-C₃N₄ was used as ECL platform and PDI-CH₃ was used as ECL label of secondary antibody(Ab₂). Increasing the CEA concentration, the loading of PDI-CH₃ on the electrode stepwisely increased, which leaded to gradual increase of ECL_PDI-CH3 at − 0.5 V and significant decrease of ECL_g-C3N4 at − 1.2 V due to the high quenching effects of PDI-CH₃ to g-C₃N₄ through dual-quenching mechanism: competitive consumption of coreactant and ECL resonance energy transfer. And the ECL_PDI-CH3/ECL_g-C3N4 showed ultrasensitive linear response for CEA between 0.1 fg mL⁻¹ and 1 ng mL⁻¹ with detection limit 0.052 fg mL⁻¹ according to IUPAC criterion. In addition, the proposed ratiometric biosensor exhibited good recovery in human serum samples.

在电化学发光(ECL)领域，电位分辨发光体的短缺严重限制了生物传感器的发展，例如比例生物传感器。在这项工作中，首次报道了N,N'-二甲基-3,4,9,10-苝二甲酰亚胺（PDI-CH ₃）和类石墨氮化碳（gC ₃ N ₄）纳米片与共反应物产生电位分辨的ECL K ₂ S ₂ O ₈介于0和-1.2 V之间。gC ₃ N ₄的ECL光谱和PDI-CH ₃的吸收光谱有很好的重叠。此外，PDI-CH ₃和 gC ₃ N ₄以gC ₃ N ₄为ECL平台，PDI-CH ₃为二抗（Ab ₂ ）的ECL标记，构建了夹层免疫传感器ECL比率系统检测肿瘤标志物癌胚抗原（CEA ）。随着 CEA 浓度的增加，电极上 PDI-CH ₃的负载量逐步增加，导致 ECL _PDI-CH3在 - 0.5 V 时逐渐增加，而 ECL _g-C3N4在 - 1.2 V 时由于高淬火效应显着降低PDI-CH ₃至 gC ₃ N ₄通过双重淬灭机制：共反应物的竞争消耗和 ECL 共振能量转移。根据 IUPAC 标准，ECL _PDI-CH3 /ECL _g-C3N4对 CEA 在 0.1 fg mL ^-1和 1 ng mL ^-1之间显示出超灵敏的线性响应，检测限为 0.052 fg mL ^{-1 。}此外，所提出的比率生物传感器在人血清样品中表现出良好的恢复。