Imidazolium ionic liquids (ILs) are chemical compounds beginning to be used on a mass scale. Although their presence in the environment is usually treated as only potential threat, there are already first evidences that this has become a real case. Taking into account their increasing use it might be expected that this problem will also increase in the nearest future. Given that some of the imidazolium cations exhibit high potential for bioconcentration, it is likely that they will accumulate in the tissues of wild organisms. Thus, there is no doubt that monitoring the presence of these compounds in organisms from potentially contaminated waters will be needed. Therefore, the aim of our study was to develop and fully validate a novel and reliable analytical procedure for the determination of the mixture of imidazolium ILs in Mytilus trossulus mussels. For this purpose, different extraction techniques were tested such as: microwave-assisted extraction (MAE), accelerated-solvent extraction (ASE) and bullet-blender homogenization (HOMO). Finally, the proposed procedure is based on the application of MAE technique for the extraction of imidazolium cations and SPE technique using Oasis HLB cartridges for the purification of the obtained extracts and LC-MS/MS technique with QqQ analyzer for their final determination. Absolute recoveries of the proposed analytical procedure reached 71–90%. The developed procedure is characterized with low limits of quantification, at 50–100 ng g⁻¹ dry tissue and allows for reliable determination of trace amounts of the tested compounds in complex biological matrix. Matrix effects obtained for the optimized procedure ranged from 7.8 to 37.5%. As a result, this is the first study presenting the analytical procedure for the analysis of imidazolium ILs in aquatic animal tissues.

咪唑鎓离子液体 (IL) 是开始大规模使用的化合物。尽管它们在环境中的存在通常被视为唯一的潜在威胁，但已经有初步证据表明这已成为真实案例。考虑到它们的使用越来越多，可以预计这个问题在不久的将来也会增加。鉴于某些咪唑阳离子表现出很高的生物富集潜力，它们很可能会在野生生物的组织中积累。因此，毫无疑问，需要监测来自潜在污染水域的生物体中这些化合物的存在。因此，我们研究的目的是开发并充分验证一种新颖可靠的分析方法，用于测定中咪唑离子液体的混合物。Mytilus trossulus贻贝。为此，测试了不同的提取技术，例如：微波辅助提取 (MAE)、加速溶剂提取 (ASE) 和子弹式搅拌机均质化 (HOMO)。最后，所提出的程序基于应用 MAE 技术提取咪唑阳离子和使用 Oasis HLB 柱纯化获得的提取物的 SPE 技术以及使用 QqQ 分析仪进行最终测定的 LC-MS/MS 技术。所提出的分析程序的绝对回收率达到 71–90%。开发的程序的特点是量化限低，为 50–100 ng g ⁻¹干组织并允许可靠地测定复杂生物基质中的痕量测试化合物。优化程序获得的基质效应范围为 7.8 至 37.5%。因此，这是第一项介绍用于分析水生动物组织中咪唑离子液体的分析程序的研究。