This study describes the development of electrochemical paper-based analytical devices (ePADs) using carbon-based paste combining silicone glue and graphite powder. The ePADs were manufactured using the screen-printing technique, which consisted of depositing the conductive ink on a screencast on the paper surface. In addition, an alternative electrical connector was designed and 3D-printed to make the detection method cheaper, portable and reproducible. The morphological, structural, and electrochemical properties of the conductive material developed were investigated through scanning electron microscopy (SEM), Raman spectroscopy, electrochemical impedance spectroscopy (EIS), and cyclic voltammetry (CV) measurements. The ePADs combined with the alternative connector revealed high repeatability, reproducibility, and stable responses considering a well-known redox probe ([Fe(CN)₆]^4-/3-). In addition, the proposed ePAD provided a linear response for standard solutions of ascorbic acid (AA) in the concentration range between 0.1 and 2.0 mmol L⁻¹. The achieved limit of detection was 4.0 μmol L⁻¹. As proof of applicability, the ePADs were evaluated for AA analysis in synthetic biofluids (blood plasma and urine), vitamin C tablets, and food (gelatine and orange juice) samples. The analytical parameters of the proposed device were compared with other reports in the literature and exhibited similar or even superior performance, highlighting its feasibility for sensing applications.

本研究描述了电化学纸基分析设备 (ePAD) 的开发，该设备使用结合了硅胶和石墨粉的碳基浆料。ePAD 是使用丝网印刷技术制造的，该技术包括将导电油墨沉积在纸表面的屏幕上。此外，还设计了一种替代电连接器并进行了 3D 打印，以使检测方法更便宜、便携和可重复。通过扫描电子显微镜 (SEM)、拉曼光谱、电化学阻抗谱 (EIS) 和循环伏安法 (CV) 测量研究了所开发的导电材料的形态、结构和电化学性能。与替代连接器相结合的 ePAD 显示出高重复性、再现性、₆ ] ^4-/3- )。^{此外，所提出的 ePAD 为 0.1 至 2.0 mmol L -1}浓度范围内的抗坏血酸 (AA) 标准溶液提供了线性响应。达到的检测限为4.0 μmol L ^-1。作为适用性的证明，我们对 ePAD 进行了评估，用于在合成生物流体（血浆和尿液）、维生素 C 片剂和食品（明胶和橙汁）样品中进行 AA 分析。该装置的分析参数与文献中的其他报告进行了比较，表现出相似甚至更优越的性能，突出了其在传感应用中的可行性。