Talanta ( IF 6.1 ) Pub Date : 2022-08-05 , DOI: 10.1016/j.talanta.2022.123770 Lifo Ruan 1 , Haijing Li 2 , Jiayu Zhang 1 , Mengxue Zhou 1 , Hui Huang 1 , Juncai Dong 2 , Jinxia Li 1 , Feng Zhao 1 , Zhonghua Wu 2 , Jun Chen 1 , Zhifang Chai 1 , Yi Hu 1
Iron oxide nanoparticles (IONPs) have been widely used as a nanoscale tool in biomedical research. However, it remains largely unknown how IONPs are transformed at a subcellular level to elicit distinct biological effects. In the present study, we prepared three different IONPs, including two IONPs targeting mitochondria (IONP-TPP) and lysosomes (IONP-APM), respectively, and a control with no specified target (IONP). By MTT assay and JC-1 staining, mitochondria-targeted IONP-TPP was found to produce significant cytotoxicity and severe mitochondrial membrane depolarization in MCF-7 cells. Furthermore, X-ray absorption spectroscopy (XAS) analysis revealed that IONP-TPP underwent remarkable edge defects and oxidation inside the cell. These findings suggest that IONPs are prone to the chemical transformation at mitochondria, and mitochondria are vulnerable to IONPs accumulation in the cell.
中文翻译:
线粒体中积累的氧化铁纳米颗粒 (IONPs) 的化学转化和细胞毒性
氧化铁纳米颗粒(IONPs)已被广泛用作生物医学研究中的纳米级工具。然而,如何在亚细胞水平上转化 IONP 以引发不同的生物学效应仍然很大程度上未知。在本研究中,我们制备了三种不同的 IONP,包括分别针对线粒体 (IONP-TPP) 和溶酶体 (IONP-APM) 的两种 IONP,以及没有指定靶标的对照 (IONP)。通过 MTT 测定和 JC-1 染色,发现线粒体靶向 IONP-TPP 在 MCF-7 细胞中产生显着的细胞毒性和严重的线粒体膜去极化。此外,X 射线吸收光谱 (XAS) 分析显示 IONP-TPP 在细胞内经历了显着的边缘缺陷和氧化。这些发现表明 IONPs 易于在线粒体发生化学转化,