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Genome-wide identification, expression and functional analysis of sugar transporters in sorghum (Sorghum bicolor L.)
Journal of Integrative Agriculture ( IF 4.8 ) Pub Date : 2022-08-01 , DOI: 10.1016/j.jia.2022.07.034
Qian-lin XIAO , Zhen LI , Ya-yun WANG , Xian-bin HOU , Xi-mei WEI , Xiao ZHAO , Lei HUANG , Yan-jun GUO , Zhi-zhai LIU

Sugar transporters are essential for osmotic process regulation, various signaling pathways and plant growth and development. Currently, few studies are available on the function of sugar transporters in sorghum (Sorghum bicolor L.). In this study, we performed a genome-wide survey of sugar transporters in sorghum. In total, 98 sorghum sugar transporters (SSTs) were identified via BLASTP. These SSTs were classified into three families based on the phylogenetic and conserved domain analysis, including six sucrose transporters (SUTs), 23 sugars will eventually be exported transporters (SWEETs), and 69 monosaccharide transporters (MSTs). The sorghum MSTs were further divided into seven subfamilies, including 24 STPs, 23 PLTs, two VGTs, four INTs, three pGlcT/SBG1s, five TMTs, and eight ERDs. Chromosomal localization of the SST genes showed that they were randomly distributed on 10 chromosomes, and substantial clustering was evident on the specific chromosomes. Twenty-seven SST genes from the families of SWEET, ERD, STP, and PLT were found to cluster in eight tandem repeat event regions. In total, 22 SSTs comprising 11 paralogous pairs and accounting for 22.4% of all the genes were located on the duplicated blocks. The different subfamilies of SST proteins possessed the same conserved domain, but there were some differences in features of the motif and transmembrane helices (TMH). The publicly-accessible RNA-sequencing data and real-time PCR revealed that the SST genes exhibited distinctive tissue specific patterns. Functional studies showed that seven SSTs were mainly located on the cell membrane and membrane organelles, and 14 of the SSTs could transport different types of monosaccharides in yeast. These findings will help us to further elucidate their roles in the sorghum sugar transport and sugar signaling pathways.



中文翻译:

高粱(Sorghum bicolor L.)糖转运蛋白的全基因组鉴定、表达和功能分析

糖转运蛋白对于渗透过程调节、各种信号通路和植物生长发育至关重要。目前,关于糖转运蛋白在高粱( Sorghum bicolor L.)中的作用的研究很少。在这项研究中,我们对高粱中的糖转运蛋白进行了全基因组调查。总共鉴定了 98 个高粱糖转运蛋白 (SST )爆炸。根据系统发育和保守域分析,这些 SST 被分为三个家族,包括 6 个蔗糖转运蛋白 (SUT)、23 个糖最终将被输出的转运蛋白 (SWEET) 和 69 个单糖转运蛋白 (MST)。高粱MSTs进一步分为7个亚科,包括24个STPs、23个PLTs、2个VGTs、4个INTs、3个pGlcT/SBG1s、5个TMTs和8个ERDs。SST基因的染色体定位显示它们随机分布在10条染色体上,并且在特定染色体上明显聚集。发现来自 SWEET、ERD、STP 和 PLT 家族的 27 个 SST 基因聚集在 8 个串联重复事件区域中。总共有22个SST,包括11对旁系同源对,占所有基因的22.4%,位于重复块上。SST蛋白的不同亚家族具有相同的保守结构域,但基序和跨膜螺旋(TMH)的特征存在一定差异。可公开访问的 RNA 测序数据和实时 PCR 显示 SST 基因表现出独特的组织特异性模式。功能研究表明,7个SSTs主要位于细胞膜和膜细胞器上,其中14个SSTs可以在酵母中转运不同类型的单糖。这些发现将有助于我们进一步阐明它们在高粱糖转运和糖信号通路中的作用。可公开访问的 RNA 测序数据和实时 PCR 显示 SST 基因表现出独特的组织特异性模式。功能研究表明,7个SSTs主要位于细胞膜和膜细胞器上,其中14个SSTs可以在酵母中转运不同类型的单糖。这些发现将有助于我们进一步阐明它们在高粱糖转运和糖信号通路中的作用。可公开访问的 RNA 测序数据和实时 PCR 显示 SST 基因表现出独特的组织特异性模式。功能研究表明,7个SSTs主要位于细胞膜和膜细胞器上,其中14个SSTs可以在酵母中转运不同类型的单糖。这些发现将有助于我们进一步阐明它们在高粱糖转运和糖信号通路中的作用。

更新日期:2022-08-01
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