Redox homeostasis contributing to the maintenance of normal cellular physiological states. On the contrary, off-kilter redox as a resolution member in the production of cardiovascular disease, rheumatoid arthritis, and even cancer. To date, a few reports have focused on ratiometric sustainable assays for ClO^- and GSH cycling due to the headache of transient reactivity, ultra-low concentrations and short lifetimes. However, methods for the design of ratiometric fluorescent probes also need to be further expanded. In this paper, we present a novel approach to the construction of ratiometric fluorescent probes via controlled modification of polysiloxane backbones. We aimed at the controlled assembly of a biocompatible, more pliable and stable “Si-O-Si” bridging framework, “red gene” perylenetetracarboxylic anhydride and “green gene” molecular sensors, successfully constructing a dual-excitation, dual-emission ratiometric fluorescent probe (PBN-1) with favorable biosafety properties, excellent photostability and promising sensitivity. PBN-1 sustainability dynamically tracked ClO^-/GSH oxidation fluctuations in HepG2 cells and zebrafish. Notably, PBN-1 distinguished dramatically the fluctuations of ClO^-/GSH in zebrafish at different growth and developmental periods. We expect that PBN-1 could provide an effective method for the design of future ratiometric fluorescent probes and expand the application of polysiloxanes in bioimaging.

氧化还原稳态有助于维持正常的细胞生理状态。相反，不平衡的氧化还原在心血管疾病、类风湿性关节炎甚至癌症的产生中作为解决成员。迄今为止，一些报告侧重于二氧化氯的比率可持续分析^-由于瞬态反应性、超低浓度和短寿命而令人头疼的 GSH 循环。然而，比率荧光探针的设计方法也需要进一步扩展。在本文中，我们提出了一种通过对聚硅氧烷主链进行可控修饰来构建比率荧光探针的新方法。我们针对生物相容性更高、更柔韧、更稳定的“Si-O-Si”桥接框架、“红色基因”苝四羧酸酐和“绿色基因”分子传感器的可控组装，成功构建了双激发、双发射比例荧光探针（PBN-1）具有良好的生物安全性、优异的光稳定性和良好的灵敏度。PBN-1可持续性动态跟踪二氧化氯^-HepG2 细胞和斑马鱼中的 /GSH 氧化波动。值得注意的是，PBN-1显着区分了斑马鱼不同生长发育时期ClO ^{- /GSH的波动。}我们期望PBN-1可以为未来比率荧光探针的设计提供一种有效的方法，并扩大聚硅氧烷在生物成像中的应用。