Clinically, butyrylcholinesterase (BChE) is an indispensable biochemical marker for evaluating liver function and organophosphorus poisoning. In this work, we proposed a novel smartphone readable colorimetry and inductively coupled plasma mass spectrometer (ICP-MS) dual-mode analytical strategy for BChE detection in clinical patients based on the phenomenon that the filter membrane can selectively separate MnO₂ nanosheets (MnO₂ NSs) and Mn²⁺. In the presence of BChE and acetylthiocholine (ATCh), the enzymatic hydrolysate (thiocholine) can efficiently trigger the decomposition of MnO₂ NSs to Mn²⁺. After selective separation of MnO₂ NSs and Mn²⁺ via the filter, colorimetric detection was achieved with a smartphone by identifying the RGB value of the MnO₂ NSs coated membrane, while the Mn²⁺ in the filtrate was detected by ICP-MS. Under optimal conditions, the limits of detection (LODs) were as low as 1.3 U/L and 0.001 U/L for the smartphone-based RGB analysis and ICP-MS assay, respectively. This method was applied to quantify BChE in 40 clinical whole blood samples of patients with clinical liver disease. The levels of BChE detected in clinical samples by this method were consistent with clinical Ellman’s colorimetric findings. Therefore, this method can provide more options for clinical cholinesterase detection and is expected to be developed into a kit.

临床上，丁酰胆碱酯酶（BChE）是评价肝功能和有机磷中毒不可缺少的生化标志物。在这项工作中，我们基于滤膜可以选择性分离MnO ₂纳米片（MnO ₂ NSs)和Mn ²⁺。在 BChE 和乙酰硫代胆碱 (ATCh) 的存在下，酶水解产物 (硫代胆碱) 可以有效地触发 MnO ₂ NSs 分解为 Mn ²⁺。通过选择性分离 MnO ₂ NSs 和 Mn ^2+后 过滤器，通过智能手机识别MnO ₂ NSs涂层膜的RGB值实现比色检测，而滤液中的Mn ²⁺通过ICP-MS检测。在最佳条件下，基于智能手机的 RGB 分析和 ICP-MS 分析的检测限 (LOD) 分别低至 1.3 U/L 和 0.001 U/L。该方法用于对 40 份临床肝病患者的临床全血样本中的 BChE 进行定量。通过这种方法在临床样本中检测到的 BChE 水平与临床 Ellman 的比色结果一致。因此，该方法可为临床胆碱酯酶检测提供更多选择，有望开发成试剂盒。