Hydrazine (N₂H₄) is carcinogenic, extremely toxic, and induces serious environmental contamination and physiological dysfunction; however, it is widely used as an industrial material. Hence, the development of a simple and effective analytical method to detect N₂H₄ detection in both environmental and biological sectors is warranted. In this work, an intramolecular charge transfer (ICT)-based fluorescent probe 1, namely (Z)− 1-(4-acetoxybenzyl)− 4-(1-cyano-2-(7-(diethylamino)− 2-oxo-2 H-chromen-3-yl)vinyl)pyridin-1-ium, was designed for dual-excitation (420 and 600 nm, excitation separations >160 nm), near infrared (NIR)-emissive, and ratiometric fluorescent detection of N₂H₄. The sensing behavior of probe 1 for N₂H₄ detection was shown to be available over a wide pH range, and detection limits of 68 nM and 569 nM were achieved at excitation wavelengths of 420 and 600 nm, respectively. In addition, probe 1 was successfully used to image mitochondrial N₂H₄ in living cells and zebrafish. Furthermore, the probe was also capable of determining hydrazine signals in test strips and environmental soil.

肼（N ₂ H ₄）具有致癌性，剧毒，会引起严重的环境污染和生理功能障碍；然而，它被广泛用作工业材料。因此，有必要开发一种简单有效的分析方法来检测环境和生物领域的 N ₂ H ₄检测。在这项工作中，基于分子内电荷转移 ( ICT)的荧光探针1，即 (Z)- 1-(4-acetoxybenzyl)- 4-(1-cyano-2-(7-(diethylamino)- 2-oxo- 2 H-chromen-3-yl)vinyl)pyridin-1-ium，设计用于双激发（420 和 600 nm，激发分离 >160 nm）、近红外 (NIR) 发射和比例荧光检测 N₂ H ₄。探针1用于 N ₂ H ₄检测的传感行为被证明可在较宽的 pH 范围内使用，并且在 420 和 600 nm 的激发波长下分别实现了 68 nM 和 569 nM 的检测限。此外，探针1已成功用于对活细胞和斑马鱼中的线粒体 N ₂ H _{4进行成像。}此外，该探针还能够确定试纸和环境土壤中的肼信号。