Achieving rapid and highly sensitive detection of biomarkers is crucial for disease diagnosis and treatment. Here, a highly sensitive and versatile dual-amplification electrochemiluminescence (ECL) biosensing platform was constructed for target detection based on DNA nanostructures and catalyzed hairpin assembly (CHA). Specifically, when the target DNA was present, it would hybridize with the auxiliary strands (D1 and D2) to form an I-shaped nanostructure, which in turn triggered the subsequent catalytic hairpin assembly reaction to generate plenty of double-stranded DNA complexes (H1–H2). The resulting double-stranded complex could be trapped on the electrode surface and adsorbed the ECL signal probe Ru(phen)₃²⁺.We found that the I-shaped nanostructure-triggered CHA reaction had higher amplification efficiency compared with traditional CHA amplification. Thus, a sensitive “signal-on” ECL biosensor was constructed for target DNA detection with a detection limit of 1.09 fM. Additionally, by combining the binding properties of C–Ag⁺–C with an elaborately designed “Ag⁺-helper” probe, the proposed strategy could be immediately utilized for the highly sensitive and selective detection of silver ions, demonstrating the versatility of the developed biosensing platform. This strategy provided a new approach with potential applications in disease diagnosis and environmental monitoring.

中文翻译：

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基于 DNA 纳米结构和催化发夹组装信号放大的多功能电化学发光生物传感平台

实现生物标志物的快速和高灵敏度检测对于疾病诊断和治疗至关重要。在这里，基于DNA纳米结构和催化发夹组装（CHA）构建了一个高灵敏度和多功能的双放大电化学发光（ECL）生物传感平台，用于目标检测。具体来说，当目标DNA存在时，它会与辅助链（D1和D2）杂交形成I形纳米结构，进而引发随后的催化发夹组装反应，生成大量双链DNA复合物（H1 –H2)。生成的双链复合物可以被捕获在电极表面并吸附 ECL 信号探针 Ru(phen) ₃ ²⁺.我们发现，与传统的CHA扩增相比，I型纳米结构触发的CHA反应具有更高的扩增效率。因此，构建了一个灵敏的“信号开启”ECL 生物传感器，用于目标 DNA 检测，检测限为 1.09 fM。^{此外，通过将 C-Ag +} -C的结合特性与精心设计的“Ag ⁺ -辅助”探针相结合，所提出的策略可以立即用于银离子的高灵敏度和选择性检测，证明了所开发的多功能性生物传感平台。该策略提供了一种在疾病诊断和环境监测中具有潜在应用的新方法。