Abstract

Osteoarthritis (OA) is a complex disorder characterized by degenerative articular cartilage in which inflammatory mechanisms play a major role in the pathogenesis. Interleukin-6 (IL6), a multifunctional cytokine, can trigger osteoclast differentiation and bone resorption. Our purpose in this study was to evaluate the association of IL-6 −174 G/C (rs1800795) and −572 G/C (rs1800796) variants with the susceptibility to OA. One hundred fifty OA patients and 150 healthy individuals were enrolled in the study. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) was used for genotyping the IL-6 gene variants. The results of analyses were evaluated for statistical significance. The pain intensity was assessed using the Visual Analogue Scale (VAS). There was a statistically significant difference in the genotype and allele frequencies of the IL-6 −174 G/C variant between patients with OA and control groups (p = 0.001, p = 0.002, respectively). IL-6 −174 G/C GG genotype and G allele were more prevalent in patients with OA. We found that the IL-6 –572 G/C variant was not different between patients and controls in either genotype distribution and allele frequency. IL-6 174 G/C and −572 G/C loci GG-GG combined genotype was significantly higher in OA patients (p = 0.00). Our study suggests that there was a strong association between the IL-6 −174 G/C variant and OA in the Turkish population. Further studies on populations of different ethnic background are necessary to prove the association of IL-6 variants with OA.

摘要

骨关节炎（OA）是一种以关节软骨退变为特征的复杂疾病，其中炎症机制在发病机制中起主要作用。白细胞介素 6 (IL6) 是一种多功能细胞因子，可触发破骨细胞分化和骨吸收。我们在本研究中的目的是评估IL-6 -174 G/C (rs1800795) 和 -572 G/C (rs1800796) 变体与 OA 易感性的关联。150 名 OA 患者和 150 名健康个体参加了该研究。聚合酶链反应限制性片段长度多态性 (PCR-RFLP) 用于对IL-6进行基因分型基因变异。评估分析结果的统计显着性。使用视觉模拟量表（VAS）评估疼痛强度。OA 患者和对照组之间IL-6 -174 G/C 变异的基因型和等位基因频率存在统计学显着差异（分别为p = 0.001，p = 0.002）。IL-6 -174 G/C GG 基因型和 G 等位基因在 OA 患者中更为普遍。我们发现IL-6 –572 G/C 变体在患者和对照之间在基因型分布和等位基因频率方面没有差异。OA 患者IL-6 174 G/C 和 -572 G/C 位点 GG-GG 联合基因型显着升高（p = 0.00）。我们的研究表明，在土耳其人群中， IL-6 -174 G/C 变体与 OA 之间存在密切关联。需要对不同种族背景的人群进行进一步研究，以证明IL-6变异与 OA 的关联。