The development of covalent organic framework nanosheet (COF NS) is becoming a vitally important research field by reason of its high permeability, ordered structure, high utilization of functional site, favourable dispersability and large aspect ratio, resulting in their widespread applications in separation, catalysis, sensing and optical device. In this work, a Tp-Bpy COF NS was prepared via an interfacial synthesis of 2,4,6-triformylphloroglucinol (Tp) and 5,5′-diamino-2,2′-bipyridine (Bpy), which has film morphology, high surface area, large pore, excellent stability and various functional site. It was utilized as a functional material to immobilize aptamers for constructing a sensitive electrochemical aptasensor. Compared with bulk Tp-Bpy COF, Tp-Bpy COF NS can significantly enhance the biosensing performance toward ultra-trace tobramycin. This work is benefit for the exploration of COF NSs and their electrochemical aptasensors in biosensing applications.

共价有机骨架纳米片（COF NS）以其高渗透性、有序结构、高功能位点利用率、良好的分散性和大的纵横比等优点，在分离、催化等领域得到广泛应用，正成为一个非常重要的研究领域。 、传感和光学器件。在这项工作中，通过界面合成 2,4,6-三甲酰间苯三酚 (Tp) 和 5,5'-二氨基-2,2'-联吡啶 (Bpy) 制备了 Tp-Bpy COF NS，其具有薄膜形态，比表面积大，孔隙大，稳定性好，功能位点多样。它被用作固定适体的功能材料，用于构建灵敏的电化学适体传感器。与bulk Tp-Bpy COF相比，Tp-Bpy COF NS可以显着提高对超微量妥布霉素的生物传感性能。