The achievement of high-throughput separation and high-sensitivity detection of complex samples has been one of the most challenging issues in the field of analytical science. The application of a single technology alone could not satisfy the above requirements. The combination of technologies with the capability of high-efficiency separation and high-sensitivity structural-recognition is highly desired to meet the technical requirements. Herein, an automatic high-performance liquid chromatography (HPLC)-surface enhanced Raman spectroscopy (SERS) hyphenated system using paper substrates as the “interface” was constructed to achieve efficient separation and real-time detection. A homogeneous Au nanoparticle was printed on the hydrophobic filter paper with the inkjet technology. The prepared substrates served as a linkage for the continuous realization of HPLC and SERS functions. The complex system was separated by HPLC, and the effluents were loaded onto automatically and continuously replaceable paper substrates for real time SERS measurements. The continuous rapid separation and real-time detection of various two-component mixtures were achieved with the separation efficiency and detection sensitivity of each technology. The results demonstrated that the HPLC-SERS hyphenated system exhibited the complementary capability of the on-line separation and continuous structural identification of illegal additives in real samples. The detection sensitivity was increased by an order of magnitude to reach 10⁻⁵ mol dm⁻³, and the efficiency and accuracy for the separation and identification on the multi-components samples were higher than those of the individual HPLC or SERS technology. It is believed that the continuous paper substrate-based HPLC-SERS hyphenated system would be developed as a promising technique for the separation and identification of multi-components mixtures with high throughput.

中文翻译：

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基于纸基连续分离检测的HPLC-SERS联用系统构建

实现复杂样品的高通量分离和高灵敏度检测一直是分析科学领域最具挑战性的问题之一。仅靠单一技术的应用无法满足上述要求。高效分离和高灵敏度结构识别技术的结合是满足技术要求的迫切需要。在此，构建了以纸基为“界面”的自动高效液相色谱（HPLC）-表面增强拉曼光谱（SERS）联用系统，以实现高效分离和实时检测。使用喷墨技术将均匀的金纳米颗粒印刷在疏水滤纸上。制备的底物作为连续实现 HPLC 和 SERS 功能的纽带。复杂系统通过 HPLC 分离，流出物被加载到自动和连续可更换的纸质基材上进行实时 SERS 测量。以每种技术的分离效率和检测灵敏度，实现了对各种双组分混合物的连续快速分离和实时检测。结果表明，HPLC-SERS联用系统表现出对真实样品中非法添加剂的在线分离和连续结构鉴定的互补能力。检测灵敏度提高了一个数量级，达到10 并且流出物被加载到自动且连续可更换的纸质基材上以进行实时SERS测量。以每种技术的分离效率和检测灵敏度，实现了对各种双组分混合物的连续快速分离和实时检测。结果表明，HPLC-SERS联用系统表现出对真实样品中非法添加剂的在线分离和连续结构鉴定的互补能力。检测灵敏度提高了一个数量级，达到10 并且流出物被加载到自动且连续可更换的纸质基材上以进行实时SERS测量。以每种技术的分离效率和检测灵敏度，实现了对各种双组分混合物的连续快速分离和实时检测。结果表明，HPLC-SERS联用系统表现出对真实样品中非法添加剂的在线分离和连续结构鉴定的互补能力。检测灵敏度提高了一个数量级，达到10 结果表明，HPLC-SERS联用系统表现出对真实样品中非法添加剂的在线分离和连续结构鉴定的互补能力。检测灵敏度提高了一个数量级，达到10 结果表明，HPLC-SERS联用系统表现出对真实样品中非法添加剂的在线分离和连续结构鉴定的互补能力。检测灵敏度提高了一个数量级，达到10^-5 mol dm ^-3，对多组分样品的分离鉴定效率和准确度均高于单独的HPLC或SERS技术。人们相信，基于连续纸基材的 HPLC-SERS 联用系统将被开发为一种有前途的技术，用于高通量分离和鉴定多组分混合物。