Iron is the main substance for maintaining life. Real-time determination of ferric ion (Fe³⁺) in living cells is of great significance for understanding the relationship of Fe³⁺ concentration changes with various physiological and pathological processes. Fluorescent probes are suitable for the detection of trace metal ions in cells due to their low toxicity and high sensitivity. In this work, a boron-dipyrromethene-based fluorescent probe (BODIPY-CL) for selective detection of Fe³⁺ was synthesized. The fluorescence emission of BODIPY-CL was determined at 516 nm. In a pH range of 1 to 10, the probe BODIPY-CL exhibits a quenching response to Fe³⁺. Meanwhile, BODIPY-CL showed a highly selective response to Fe³⁺ compared with 16 kinds of metal ions. The stoichiometry ratio of BODIPY-CL bound to Fe³⁺ was nearly 2 : 1. The fluorescence quenching response obtained by the sensor was linear with the Fe³⁺ concentration in the range of 0–400 μM, and the detection limit was 2.9 μM. BODIPY-CL was successfully applied to image Fe³⁺ in cells. This study provides a promising fluorescent imaging probe for further research on the physiological and pathological effects of Fe³⁺.

中文翻译：

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基于 BODIPY 的 Fe3+ 荧光猝灭探针的合成和生物成像

铁是维持生命的主要物质。^{活细胞中铁离子(Fe 3+} )的实时测定对于了解Fe ³⁺浓度变化与各种生理病理过程的关系具有重要意义。荧光探针具有毒性低、灵敏度高等特点，适用于细胞内痕量金属离子的检测。在这项工作中，合成了一种用于选择性检测Fe ³⁺的基于硼-二吡咯亚甲基的荧光探针（BODIPY-CL ） 。BODIPY-CL的荧光发射在 516 nm 处测定。在 1 到 10 的 pH 值范围内，探针BODIPY-CL对 Fe ³⁺表现出猝灭响应。同时，与16种金属离子相比，BODIPY-CL对Fe ³⁺表现出高度选择性响应。BODIPY-CL与Fe ³⁺结合的化学计量比接近2:1。传感器获得的荧光猝灭响应与Fe ³⁺浓度在0-400 μM范围内呈线性关系，检测限为2.9 μM . BODIPY-CL成功应用于细胞内Fe ^3+成像。本研究为进一步研究Fe ³⁺的生理和病理作用提供了一种很有前景的荧光成像探针。