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Preparation of 3D Printing PLGA Scaffold with BMP-9 and P-15 Peptide Hydrogel and Its Application in the Treatment of Bone Defects in Rabbits
Contrast Media & Molecular Imaging ( IF 3.009 ) Pub Date : 2022-07-31 , DOI: 10.1155/2022/1081957
Xiaomei Wang 1 , Wanjun Chen 2 , Zhe Chen 1 , Yixiu Li 3 , Kai Wu 1 , Yulin Song 1
Affiliation  

Objective. To prepare a three-dimensional (3D) printing polylactic acid glycolic acid (PLGA) scaffold with bone morphogenetic protein-9 (BMP-9) and P-15 peptide hydrogel and evaluate its application in treating bone defects in rabbits. Methods. 3D printing PLGA scaffolds were formed and scanned by electron microscopy. Their X-ray diffraction (XRD), in vitro degradation, and compressive strength were characterized. BMP-9 and P-15 hydrogels were prepared. Flow cytometry was used to detect apoptosis, and an electron microscope was used to evaluate cell adhesion to scaffolds. Alkaline phosphatase (ALP), type 1 collagen (Col-I), osteocalcin (OCN), runt-related transcription factor 2 (RUNX2), and osterix (SP7) were detected by western blotting. MicroCT was used to detect new bone formation, and bone tissue-related protein expressions were determined in the rabbit model with bone defects. Results. The 3D printing scaffolds were cylindrical, and the inner diameter of the scaffolds was about 1 mm. The bread peak with wide distribution showed that the 3D printing only involved a physical change, which did not change the properties of the materials. The degradation rate of scaffolds was 9.38%, which met the requirements of properties of biological scaffolds. The water absorption of the support was about 9.09%, and the compressive strength was 15.83 N/mm2. In the coculture of bone marrow mesenchymal stem cells (BMSCs) with scaffolds, the 2% polypeptide hydrogel showed the most obvious activity in promoting the differentiation of BMSCs. Flow cytometry showed that the 0% and 2% groups did not cause obvious apoptosis compared with the control group. Scaffolds with 2% and 4% polypeptide promoted the expression of ALP, COL-1, OCN, RUNX2, and Sp7 in BMSCs. In vivo experiments showed that the expression of ALP, COL-1, OCN, RUNX2, and Sp7 protein in the 2% polypeptide scaffold group increased significantly compared with the model group. MicroCT detection demonstrated that the 2% polypeptide scaffold had good bone repair ability. Conclusion. The PLGA scaffolds combined with BMP-9 and P-15 peptide hydrogels had good biological and mechanical properties and could repair bone defects in rabbits.

中文翻译:

BMP-9和P-15肽水凝胶3D打印PLGA支架的制备及其在兔骨缺损治疗中的应用

客观。以骨形态发生蛋白9(BMP-9)和P-15肽水凝胶制备三维(3D)打印聚乳酸乙醇酸(PLGA)支架并评价其在治疗兔骨缺损中的应用。方法。形成 3D 打印 PLGA 支架并通过电子显微镜扫描。它们的 X 射线衍射 (XRD),体外降解和抗压强度进行了表征。制备了 BMP-9 和 P-15 水凝胶。流式细胞仪用于检测细胞凋亡,电子显微镜用于评估细胞对支架的粘附。通过蛋白质印迹检测碱性磷酸酶 (ALP)、1 型胶原蛋白 (Col-I)、骨钙素 (OCN)、runt 相关转录因子 2 (RUNX2) 和osterix (SP7)。MicroCT用于检测新骨形成,并在有骨缺损的兔模型中测定骨组织相关蛋白的表达。结果. 3D打印支架呈圆柱形,支架内径约为1 mm。分布较广的面包峰表明,3D打印只涉及物理变化,并没有改变材料的性质。支架降解率为9.38%,符合生物支架性能要求。支撑体的吸水率约为9.09%,抗压强度为15.83 N/mm 2. 在骨髓间充质干细胞(BMSCs)与支架的共培养中,2%多肽水凝胶在促进BMSCs分化方面表现出最明显的活性。流式细胞仪显示,0%和2%组与对照组相比均未引起明显的细胞凋亡。含有 2% 和 4% 多肽的支架促进了 BMSCs 中 ALP、COL-1、OCN、RUNX2 和 Sp7 的表达。体内实验表明,2%多肽支架组ALP、COL-1、OCN、RUNX2、Sp7蛋白的表达较模型组显着升高。MicroCT检测表明2%多肽支架具有良好的骨修复能力。结论. PLGA支架结合BMP-9和P-15肽水凝胶具有良好的生物学和力学性能,可以修复兔骨缺损。
更新日期:2022-07-31
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