The main objective of the current study is to establish an on-chip strategy to analyze the allergens existing in the myofibrillar proteins of seafood matrices. On-chip immunomagnetic separation (IMS) of allergens derived from large yellow croaker (Larimichthys crocea), swimming crab (Portunus trituberculatus) and black tiger shrimp (Penaeus monodon), as model seafoods, was studied. The analysis of the tryptic digests of on-chip captured proteins, combined with protein database searches, was performed for the identification of allergens by MALDI-TOF MS with a 0% false-positive rate. By using the sera of ten seafood-allergic patients, six allergens were identified from the myofibrillar proteins of the large yellow croaker. The present protocol was successfully validated by ELISA and can be applied in blood tests for food allergies with a single drop of blood. The current protocol is presumed to be applied in the identification of potential allergens in other organisms.

中文翻译：

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用于快速过敏测试的海鲜肌原纤维蛋白过敏原的芯片免疫磁分离

本研究的主要目的是建立一种芯片策略来分析海鲜基质肌原纤维蛋白中存在的过敏原。大黄鱼 ( Larimichthys crocea )、梭子蟹 ( Portunus trituberculatus ) 和黑虎虾 ( Penaeus monodon ) 过敏原的芯片免疫磁分离 (IMS))，作为模型海鲜，进行了研究。对芯片上捕获的蛋白质的胰蛋白酶消化物进行分析，结合蛋白质数据库搜索，通过 MALDI-TOF MS 以 0% 的假阳性率鉴定过敏原。通过使用十名海鲜过敏患者的血清，从大黄鱼的肌原纤维蛋白中鉴定出六种过敏原。本协议已通过 ELISA 成功验证，可用于单滴血食物过敏的血液测试。目前的协议被假定用于识别其他生物体中的潜在过敏原。