This study reveals a high level of behavioral heterogeneity in engineered T cells that target tumor organoids. Behavior-guided transcriptomics identified the gene signatures of T cells with highly potent serial cytotoxicity. Integration of the organoid and T cell transcriptomic data enabled the design of patient-specific combinatorial treatment to achieve an optimized response to cancer immunotherapies.

中文翻译：

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BEHAV3D：揭示 T 细胞抗肿瘤活性的成像和转录组学平台

这项研究揭示了靶向肿瘤类器官的工程化 T 细胞的高度行为异质性。行为引导的转录组学确定了具有高效连续细胞毒性的 T 细胞的基因特征。类器官和 T 细胞转录组数据的整合使患者特异性组合治疗的设计能够实现对癌症免疫疗法的优化反应。