Abstract

To promote the exploitation of unconventional oil resources by indigenous microorganisms, the bacterial community profiles of oil shale and sandstone in Ordos Basin were investigated using Illumina Miseq sequencing combined with the culture-based method, which was performed and reported in this literature for the first time. A total of 601 operational taxonomic units (OTUs) were obtained from collected samples, the predominant phylum present in all samples was Proteobacteria (76.96%-93.07%). Discriminatory bacterial community profiles existed in those samples by culture-dependent and culture-independent methods, with variations not only in diversity indices but also in the abundance of bacteria at different genus levels. The dominant genera in cultured sandstone sample (SCB), uncultured sandstone sample (SUB), cultured shale sample (YCB), uncultured shale sample (YUB) were Enhydrobacter (71.62%), Acidovorax (42.44%), Pseudomonas (40.13%), Variovorax (70.02%), respectively. Both sample sources and culturing methods were the principal factors affecting the variation, while the communities’ structures were favored primarily by culture-dependent or culture-independent approaches. The high abundance of hydrocarbon degradation-related genes was exhibited in YCB, which reveals a great potential for utilization of the culture-dependent method in shale oil exploitation. This study provided guidance for the exploitation of shale oil and sandstone oil by artificial utilization of indigenous bacteria.

摘要

为促进本土微生物对非常规石油资源的开发利用，采用Illumina Miseq测序技术结合培养方法对鄂尔多斯盆地油页岩和砂岩的细菌群落特征进行了研究，并在该文献中首次进行报道。 . 从收集的样本中获得了总共 601 个操作分类单位（OTU），所有样本中存在的优势门是变形杆菌门(76.96%-93.07%)。通过依赖培养和不依赖培养的方法，这些样本中存在歧视性的细菌群落分布，不仅多样性指数存在差异，而且不同属水平的细菌丰度也存在差异。培养砂岩样品（SCB）、未培养砂岩样品（SUB）、培养页岩样品（YCB）、未培养页岩样品（YUB）的优势菌属为水杆菌属（ 71.62 %）、嗜酸菌属（42.44%）、假单胞菌属（40.13%）、杂食性动物（70.02%），分别。样本来源和培养方法都是影响变异的主要因素，而社区结构主要受文化依赖或文化独立方法的青睐。在YCB中表现出高丰度的烃降解相关基因，这揭示了培养依赖方法在页岩油开采中的巨大潜力。该研究为人工利用本土细菌开发页岩油和砂岩油提供了指导。