The environmental problem posed by shale gas wastewater has received considerable research attention. Researchers mostly focus on the technical aspect of terminal centralized treatment with little concern on the topic of early collection and on-site pretreatment of PW near gas gathering stations. In this study, the short-term fluctuations of the DOM molecular composition and the concurrent transformation of the microbial community and functions were carefully examined when the PW was temporarily stored in a simulated reservoir near gas wells. Ultra-high resolution mass spectrometry was used to determine the molecular structure of the DOM samples. The data showed that during the short-term storage of PW, strongly polarized and nonvolatile organic compounds developed a complex molecular structure, resulting in molecules that were more unsaturated and lower bioavailability. Microbial activity was responsible for the changes in organic matter composition that occurred in the later stages of the storage process, during which the amount of nitrogenous organic molecules increased. Network analysis offered vital information on microbial functions related to organic matter composition and the patterns of interaction between them. Organic molecule consumption and accumulation were intimately connected to the interactions between various bacteria the same as the consumption and accumulation of DOM molecules.

页岩气废水所带来的环境问题引起了广泛的研究关注。研究人员主要关注终端集中处理的技术方面，很少关注集气站附近PW的早期收集和现场预处理问题。在本研究中，DOM 分子组成的短期波动和微生物群落的同步转化当 PW 临时储存在气井附近的模拟储层中时，仔细检查了其功能和功能。超高分辨率质谱用于确定 DOM 样品的分子结构。数据表明，在 PW 的短期储存过程中，强极化和非挥发性有机化合物发展出复杂的分子结构，导致分子不饱和度更高，生物利用度更低。微生物活动是导致储存过程后期发生的有机物质组成变化的原因，在此期间含氮有机分子的数量增加。网络分析提供了有关与有机物组成相关的微生物功能以及它们之间相互作用模式的重要信息。有机分子的消耗和积累与各种细菌之间的相互作用密切相关，就像DOM分子的消耗和积累一样。