DNA-templated copper nanoclusters (CuNCs) have limited applications because of their low fluorescence stability (several tens of minutes). In this study, we prepared CuNCs with improved temporal fluorescence stability by introducing fructose into the CuNC synthesis process and optimizing the reaction conditions. The inclusion of fructose increased the operating lifetime of CuNCs by approximately 5200-fold from 30 min to 108 days and improved their stability against heat, acids, and bases compared to CuNCs synthesized under original conditions. In addition, the fluorescence signal of CuNCs was maintained for a significantly longer time when stored at refrigeration (4 °C) and freezing (−20 °C) temperatures. Importantly, this method did not require the addition of substances other than fructose or any additional physicochemical treatment to maintain the fluorescence of DNA-templated CuNCs for more than several tens of days. As such, this study could serve as a basis to improve the stability of CuNCs for various applications.

中文翻译：

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高果糖浓度将 DNA 模板铜纳米簇的荧光稳定性提高数千倍

以 DNA 为模板的铜纳米簇 (CuNC) 由于荧光稳定性低（几十分钟），因此应用有限。在本研究中，我们通过在 CuNC 合成过程中引入果糖并优化反应条件，制备了具有改善的时间荧光稳定性的 CuNCs。与在原始条件下合成的 CuNCs 相比，果糖的加入使 CuNCs 的使用寿命从 30 分钟增加到 108 天，增加了大约 5200 倍，并提高了它们对热、酸和碱的稳定性。此外，在冷藏（4°C）和冷冻（-20°C）温度下储存时，CuNCs 的荧光信号可以保持更长的时间。重要的，这种方法不需要添加果糖以外的物质或任何额外的物理化学处理来维持 DNA 模板 CuNCs 的荧光超过数十天。因此，这项研究可以作为提高CuNCs在各种应用中的稳定性的基础。