Chemiluminescence-based detection methods offer a superior signal-to-noise ratio and are commonly adopted for biosensors. This work presents the design and implementation of a supramolecular assay based on a chemiluminescent chemosensor. Specifically, an indicator displacement assay (IDA) with the supramolecular host–guest complex of chemiluminescent phenoxy 1,2-dioxetane and cucurbit[8]uril enables the low-micromolar detection of drugs in human urine and human serum samples. Cucurbit[8]uril thereby acts as a non-surfactant chemiluminescence enhancer and a synthetic receptor. Additionally, we show that adding an equimolar amount of cucurbit[8]uril to a commercially available dioxetane used in standard enzymatic chemiluminescence immunoassays enhances the chemiluminescence by more than 15 times. Finally, we demonstrate that a chemiluminescence resonance energy transfer between a unimolecular macrocyclic cucurbit[7]uril-dye conjugate and a phenoxy 1,2-dioxetane can be utilized to detect the herbicide paraquat at a micromolar concentration in aqueous media.

中文翻译：

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基于葫芦[n]脲的化学发光化学传感器用于检测生物流体中的药物

基于化学发光的检测方法具有出色的信噪比，通常用于生物传感器。这项工作介绍了基于化学发光化学传感器的超分子检测的设计和实施。具体而言，使用化学发光苯氧基 1,2-二氧杂环丁烷和葫芦[8]脲的超分子主客体复合物进行指示剂置换分析 (IDA)，可以检测人尿液和人血清样品中的低微摩尔浓度药物。葫芦[8]脲因此充当非表面活性剂化学发光增强剂和合成受体。此外，我们表明，在标准酶化学发光免疫测定中使用的市售二氧杂环丁烷中加入等摩尔量的葫芦[8]脲可使化学发光增强 15 倍以上。最后，