Textile azo dyes are used for coloring fabrics which are found to be potentially carcinogenic and mutagenic chemicals. It is disposed of as industrial effluents, causing major hazardous impacts on the aquatic ecosystem and getting deposited onto the soil for a prolonged period. Several conventional methods have been previously applied to eliminate these dyes but had low efficiency and high cost limitations. In order to overcome those issues, in this study, we have synthesized a GO–TiO₂ nanocomposite using the solvothermal method from graphene oxide (GO) nanoparticles and titanium dioxide (TiO₂) nanoparticles which were synthesized individually using modified Hummer’s method and sol–gel method, respectively. The prepared TiO₂ nanoparticles and GO–TiO₂ nanocomposite were characterized using UV–visible spectrophotometry, FTIR spectroscopy, dynamic light scattering, zeta potential, XRD, scanning electron microscopy, and EDAX analysis. The textile azo dyes used were Crystal Violet, Brilliant Green, Malachite Green, and Rhodamine B, and their photocatalytic degradation was observed spectrophotometrically using TiO₂ nanoparticles and GO–TiO₂ nanocomposite. From the results obtained, we found that the GO–TiO₂ nanocomposite was successfully synthesized with TiO₂ doping and effectively reduced all the dyes used in this study compared to the standard photocatalyst TiO₂. On the other hand, the Brilliant Green and Malachite green have shown an increase in their color after TiO₂ treatment, which was diminished by using GO–TiO₂ nanocomposites. The biocompatibility of this GO–TiO₂ was studied using zebrafish embryos and the results demonstrated that the GO–TiO₂ nanocomposite was safe for the embryos without causing any delay in hatching or developmental abnormality. The outcome of this study yields a novel application of GO–TiO₂ nanocomposite in the reduction in azo dyes showing potential applications in wastewater remediation. Further studies are required to test the biocompatibility and efficacy of other dye removals.

纺织偶氮染料用于为被发现具有潜在致癌和致突变化学物质的织物着色。它作为工业废水处理，对水生生态系统造成重大危险影响，并长期沉积在土壤中。以前已经应用了几种常规方法来消除这些染料，但效率低且成本高。为了克服这些问题，在本研究中，我们使用溶剂热法从氧化石墨烯 (GO) 纳米颗粒和二氧化钛 (TiO _{2 ) 纳米颗粒合成了 GO-TiO 2}纳米复合材料，它们分别使用改进的 Hummer 方法和溶胶-凝胶法，分别。制备的 TiO ₂纳米粒子和 GO-TiO使用紫外-可见分光光度法、FTIR 光谱、动态光散射、zeta 电位、XRD、扫描电子显微镜和 EDAX 分析对_{2纳米复合材料进行了表征。}使用的纺织偶氮染料是水晶紫、亮绿、孔雀绿和罗丹明B，并使用TiO ₂纳米颗粒和GO-TiO ₂纳米复合材料通过分光光度法观察它们的光催化降解。从获得的结果中，我们发现，与标准光催化剂 TiO ₂相比， GO-TiO ₂纳米复合材料成功地合成了 TiO _{2掺杂，并有效地减少了本研究中使用的所有染料。}. 另一方面，亮绿和孔雀绿在 TiO _{2处理后显示出颜色的增加，而使用 GO-TiO 2}纳米复合材料则减少了颜色。使用斑马鱼胚胎研究了这种 GO-TiO ₂的生物相容性，结果表明 GO-TiO ₂纳米复合材料对胚胎是安全的，不会导致孵化延迟或发育异常。这项研究的结果产生了 GO-TiO ₂纳米复合材料在减少偶氮染料中的新应用，显示出在废水修复中的潜在应用。需要进一步的研究来测试其他染料去除剂的生物相容性和功效。