During biological detection, the toxicity caused by probes to living organisms is neglected. In this study, an analyte-compensated fluorescent probe (NP-SN₃) was constructed for the detection of H₂S. Through experiments with HepG2 cells and zebrafish embryos and larvae, the NP-SN₃ probe showed no significant difference in imaging performance compared with the traditional probe (NP-N₃) but exhibited lower detection-induced toxicity in the imaging of liver fibrosis in activated HSC-T6 cells. During the development of zebrafish embryos and continuous administration in rats, NP-SN₃ showed a lower death rate, higher hatchability and lower malformation in zebrafish embryos and milder pathological symptoms in stained rat tissues.

中文翻译：

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分析物补偿荧光探针的设计策略以降低其毒性

在生物检测过程中，忽略了探针对生物体的毒性。本研究构建了一种用于检测H _{2 S的分析物补偿荧光探针（NP-SN 3} ） 。通过HepG2细胞和斑马鱼胚胎和幼虫的实验，NP-SN ₃探针的成像性能没有显着差异与传统探针（NP-N ₃）相比，但在活化的 HSC-T6 细胞肝纤维化成像中表现出较低的检测诱导毒性。在斑马鱼胚胎发育和大鼠连续给药期间，NP-SN ₃在斑马鱼胚胎中表现出较低的死亡率、较高的孵化率和较低的畸形率，在染色的大鼠组织中表现出较轻的病理症状。