Enzymatic ligation is a popular method in DNA nanotechnology for structural enforcement. When employed as stability switch for chosen components, ligation can be applied to induce DNA nanostructure reconfiguration. In this study, we investigate the reinforcement effect of ligation on addressable DNA nanostructures assembled entirely from short synthetic strands as the basis of structural reconfiguration. A careful calibration of ligation efficiency is performed on structures with programmable nicks. Systematic investigation using comparative agarose gel electrophoresis enables quantitative assessment of enhanced survivability with ligation treatment on a number of unique structures. The solid ligation performance sets up the foundation for the ligation-based structural reconfiguration. With the capability of switching base pairing status between permanent and transient (ON and OFF) by a simple round of enzymatic treatment, ligation induced reconfiguration can be engineered for DNA nanostructures accordingly.

中文翻译：

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酶促连接处理诱导的 DNA 纳米结构重构

酶促连接是 DNA 纳米技术中用于结构强化的一种流行方法。当用作选定组件的稳定性开关时，可以应用连接来诱导 DNA 纳米结构重新配置。在这项研究中，我们研究了连接对完全由短合成链组装而成的可寻址 DNA 纳米结构的增强作用，作为结构重构的基础。在具有可编程刻痕的结构上执行连接效率的仔细校准。使用比较琼脂糖凝胶电泳的系统研究能够定量评估在许多独特结构上通过连接处理提高的存活率。扎实的结扎性能为基于结扎的结构重构奠定了基础。