Microbiota colonize every accessible plant tissue and play fundamental roles in plant growth and health. Soybean stay-green syndrome (SGS), a condition that causes delayed leaf senescence (stay-green), flat pods and abnormal seeds of soybean, has become the most serious disease of soybean in China. However, the direct cause of SGS is highly debated, and little is known about how SGS affect soybean microbiome dynamics, particularly the seed microbiome. We studied the bacterial, fungal, and viral communities associated with different soybean tissues with and without SGS using a multi-omics approach, and investigated the possible pathogenic agents associated with SGS and how SGS affects the assembly and functions of plant-associated microbiomes. We obtained a comprehensive view of the composition, function, loads, diversity, and dynamics of soybean microbiomes in the rhizosphere, root, stem, leaf, pod, and seed compartments, and discovered that soybean SGS was associated with dramatically increased microbial loads and dysbiosis of the bacterial microbiota in seeds. Furthermore, we identified a novel geminivirus that was strongly associated with soybean SGS, regardless of plant cultivar, sampling location, or harvest year. This whole-plant microbiome profiling of soybean provides the first demonstration of geminivirus infection associated with microbiota dysbiosis, which might represent a general microbiological symptom of plant diseases.

中文翻译：

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全植物微生物组分析揭示了一种与大豆保持绿色病害相关的新型双子病毒

微生物群定植于每一个可接近的植物组织，并在植物生长和健康中发挥重要作用。大豆滞青综合征（SGS）是一种导致大豆叶片衰老延迟（滞青）、扁豆荚和异常种子的病症，已成为我国大豆最严重的病害。然而，SGS 的直接原因备受争议，关于 SGS 如何影响大豆微生物组动态，尤其是种子微生物组，我们知之甚少。我们使用多组学方法研究了与有和没有 SGS 的不同大豆组织相关的细菌、真菌和病毒群落，并研究了与 SGS 相关的可能病原体以及 SGS 如何影响植物相关微生物组的组装和功能。我们全面了解了组成、功能、负载、多样性、根际、根、茎、叶、豆荚和种子区室中大豆微生物群落的动态和动态，并发现大豆 SGS 与种子中细菌微生物群的微生物负荷和生态失调显着增加有关。此外，我们发现了一种与大豆 SGS 密切相关的新型双子病毒，无论植物品种、采样地点或收获年份如何。这种对大豆的全植物微生物组分析首次证明了与微生物群失调相关的双子病毒感染，这可能代表了植物病害的一般微生物学症状。我们发现了一种与大豆 SGS 密切相关的新型双子病毒，无论植物品种、采样地点或收获年份如何。这种对大豆的全植物微生物组分析首次证明了与微生物群失调相关的双子病毒感染，这可能代表了植物病害的一般微生物学症状。我们发现了一种与大豆 SGS 密切相关的新型双子病毒，无论植物品种、采样地点或收获年份如何。这种对大豆的全植物微生物组分析首次证明了与微生物群失调相关的双子病毒感染，这可能代表了植物病害的一般微生物学症状。