A phenyl-based monolithic adsorbent was prepared in a 50-mm-long stainless steel tube, which was initiated by the redox system, using ethylene glycol phenyl ether acrylate as the monomer and ethylene glycol dimethacrylate as the crosslinker. The effects of monomer/crosslinker ratio and the porogens on the permeability and morphology of the resulting adsorbents were investigated, and the optimal adsorbent shows relatively uniform pore structure according to the characterizations of scanning electron microscopy and nitrogen adsorption–desorption method. The column that filled with the adsorbent was used as the solid-phase extraction (SPE) cartridge, exhibiting unique selectivity for the extraction of evodiamine from Euodia fructus (the fruits of Euodia rutaecarpa (Juss.)Benth.), which attributes to the interactions of π-π and hydrogen bonding between the adsorbent and evodiamine. Combined with a C18 analytical column via high-performance liquid chromatography (HPLC) system, an online SPE-HPLC method was established for extraction, enrichment and determination of evodiamine from Euodia fructus. Method validation demonstrates that the relative standard deviation of the precision is less than 0.66%, and the spiked recovery is in the range of 93.11–98.06%. Furthermore, it is worth noting that the prepared SPE cartridge can be reused for no less than 100 times. These results show that the developed method is simple and efficient for online extraction and enrichment of evodiamine from Euodia fructus.

中文翻译：

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基于自制苯基整体柱的SPE-HPLC法提取并测定吴茱萸中吴茱萸碱的含量

以乙二醇苯醚丙烯酸酯为单体、乙二醇二甲基丙烯酸酯为交联剂，通过氧化还原体系引发，在50 mm长的不锈钢管中制备了苯基整体式吸附剂。研究了单体/交联剂比例和致孔剂对所得吸附剂的渗透性和形貌的影响，根据扫描电子显微镜和氮气吸附-解吸法的表征，最佳吸附剂显示出相对均匀的孔结构。填充吸附剂的色谱柱用作固相萃取（SPE）柱，对从吴茱萸（Euodia rutaecarpa (Juss.)Benth.）的果实中提取吴茱萸碱表现出独特的选择性，这归因于吸附剂和吴茱萸碱之间的π-π相互作用和氢键作用。结合C18分析柱和高效液相色谱（HPLC）系统，建立了吴茱萸中吴茱萸碱的提取、富集和测定的在线SPE-HPLC方法。方法验证表明，精密度的相对标准偏差小于0.66%，加标回收率在93.11%~98.06%之间。此外，值得注意的是，制备的SPE小柱可重复使用不少于100次。这些结果表明，所开发的方法简单有效，用于在线提取和富集吴茱萸中吴茱萸碱。建立了吴茱萸中吴茱萸碱的在线提取、富集和测定方法。方法验证表明，精密度的相对标准偏差小于0.66%，加标回收率在93.11%~98.06%之间。此外，值得注意的是，制备的SPE小柱可重复使用不少于100次。这些结果表明，所开发的方法简单有效，用于在线提取和富集吴茱萸中吴茱萸碱。建立了吴茱萸中吴茱萸碱的在线提取、富集和测定方法。方法验证表明，精密度的相对标准偏差小于0.66%，加标回收率在93.11%~98.06%之间。此外，值得注意的是，制备的SPE小柱可重复使用不少于100次。这些结果表明，所开发的方法简单有效，用于在线提取和富集吴茱萸中吴茱萸碱。值得注意的是，制备好的SPE小柱可重复使用不少于100次。这些结果表明，所开发的方法简单有效，用于在线提取和富集吴茱萸中吴茱萸碱。值得注意的是，制备好的SPE小柱可重复使用不少于100次。这些结果表明，所开发的方法简单有效，用于在线提取和富集吴茱萸中吴茱萸碱。