Cofactor NADPH is widely used in enzymatic redox reactions, however, its regeneration by glucose dehydrogenase (GDH) suffers from massive glucose consumption, costly wastewater treatment, and low atom efficiency. Herein, Fe₃O₄ nanoparticles supported on helical multi-walled carbon nanotubes (Fe₃O₄@HMWCNTs) was prepared and displayed comparable transfer hydrogenation activity to Exiguobacterium sibiricum GDH (EsGDH). We developed a chemoenzymatic catalysis based on a combination of an aldo–keto reductase KmAKR with Fe₃O₄@HMWCNTs scaffold, which asymmetrically synthesized optically pure tert-butyl 6-cyano-(3R,5R)-dihydroxyhexanoate ((3R,5R)-2, > 99.5 % de_p). Under the optimized conditions, 10 mM NADP⁺ was converted to NADPH by 5.0 mg Fe₃O₄@HMWCNTs, in a yield of > 80.0 %. The combination of Fe₃O₄@HMWCNTs with KmAKR_M7 completely reduced 10 mM tert-butyl 6-cyano-(5R)-hydroxy-3-oxohexanoate ((5R)-1) to (3R,5R)-2, in a conversion of > 99.0 % and de_p > 99.5 %. Contrast to the previous developed biocatalytic approach with KmAKR and EsGDH, this efficient and compatible nanozyme-enzyme process required neither glucose/alcohols as co-substrate nor a glucose dehydrogenase/alcohol dehydrogenase for NADPH regeneration.

辅因子 NADPH 广泛用于酶促氧化还原反应，但其通过葡萄糖脱氢酶 (GDH) 再生存在大量葡萄糖消耗、废水处理成本高和原子效率低等问题。在此，制备了负载在螺旋状多壁碳纳米管（Fe ₃ O ₄ @HMWCNTs）上的 Fe ₃ O _{4纳米颗粒，并显示出与 Exiguobacterium} sibiricum GDH ( Es GDH) 相当的转移氢化活性。我们开发了一种基于醛酮还原酶Km AKR 与 Fe ₃ O ₄ @HMWCNTs 支架组合的化学酶催化，该支架不对称地合成了光学纯叔-6-氰基-(3 R ,5 R )-二羟基己酸丁酯 ((3 R ,5 R )- 2 , > 99.5 % de _p )。在优化条件下，10 mM NADP ⁺通过 5.0 mg Fe ₃ O ₄ @HMWCNTs 转化为 NADPH，产率 > 80.0 %。Fe ₃ O ₄ @HMWCNTs 与Km AKR _M7的组合将 10 mM 6-氰基-(5 R )-羟基-3-氧代己酸叔丁酯 ((5 R )- 1 ) 完全还原为 (3 R ,5 R ) - 2, 转化率 > 99.0 % 和de _p  > 99.5 % 。与之前开发的Km AKR 和Es GDH 生物催化方法相比，这种高效且相容的纳米酶-酶过程既不需要葡萄糖/醇作为共底物，也不需要葡萄糖脱氢酶/醇脱氢酶来再生 NADPH。