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Genetic mapping of sugarcane aphid resistance in sorghum line SC112-14
Crop Science ( IF 2.3 ) Pub Date : 2022-07-20 , DOI: 10.1002/csc2.20818
Hugo E. Cuevas 1 , Joseph E. Knoll 2 , Karen R. Harris‐Shultz 2 , Somashekhar M. Punnuri 3
Affiliation  

Sugarcane aphid [Melanaphis sacchari (Zehntner)] is a destructive pest that has had an economic effect on sorghum in North America since 2013. The identification, development, and use of resistant sorghum germplasm is the most feasible strategy to control the pest. Nevertheless, the genetic control of sugarcane aphid (SCA) resistance is unknown for most sorghum resistant lines. To identify the genetic regions that confer SCA resistance in sorghum line SC112-14, 103 recombinant inbred lines (RILs) derived by its cross with the susceptible line PI 609251 were evaluated for their SCA resistance response in Georgia during two consecutive years. The resistance response was determined based on two ratings (2 wk apart) for aphid population size (APS) and aphid-induced plant damage (APD) each year. Segregation for SCA resistance was observed for the first APS and both APD ratings, and the broad-sense heritability estimate ranged from .71 to .76, respectively. A quantitative trait locus analysis using a high-density linkage map of 3,852 single nucleotide polymorphisms (SNPs) detected an 81-kb genomic region on chromosome 6 that explained 50–55% of the phenotypic variation. Comparative mapping analysis found that the resistance locus in SC112-14 is located 8- and 10-cM upstream of the Henong 16 (RMES1) and Tx2783 resistance loci, respectively, and encloses the SNP Sbv3.1_06_2316351 associated in Haitian resistant lines. Therefore, the line SC112-14 is an additional SCA resistance source that can be combined or strategically used with other resistance sources to assure a more robust host plant resistance to the SCA.

中文翻译:

高粱品系SC112-14抗甘蔗蚜虫遗传图谱

甘蔗蚜虫 [ Melanaphis sacchari(Zehntner)]是一种破坏性害虫,自 2013 年以来对北美高粱产生了经济影响。鉴定、开发和使用抗性高粱种质资源是控制该害虫最可行的策略。然而,对于大多数高粱抗性品系,甘蔗蚜虫 (SCA) 抗性的遗传控制尚不清楚。为了鉴定赋予高粱品系 SC112-14 SCA 抗性的遗传区域,在格鲁吉亚连续两年评估了由其与易感品系 PI 609251 杂交衍生的 103 个重组近交系 (RIL) 的 SCA 抗性反应。抗性反应是根据每年对蚜虫种群规模 (APS) 和蚜虫引起的植物损害 (APD) 的两个评级(相隔 2 周)确定的。对于第一个 APS 和两个 APD 评级,观察到 SCA 抗性的分离,和广义遗传力估计范围分别为 0.71 至 0.76。使用 3,852 个单核苷酸多态性 (SNP) 的高密度连锁图谱进行数量性状位点分析,检测到 6 号染色体上一个 81-kb 的基因组区域,该区域解释了 50-55% 的表型变异。比较作图分析发现,SC112-14 的抗性位点位于河农 16 号上游 8 和 10-cM 处(RMES1 ) 和 Tx2783 抗性基因座,并包含与海地抗性品系相关的 SNP Sbv3.1_06_2316351。因此,SC112-14 系是一种额外的 SCA 抗性来源,可以与其他抗性来源组合或有策略地使用,以确保寄主植物对 SCA 具有更强大的抗性。
更新日期:2022-07-20
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