Kidney International ( IF 19.6 ) Pub Date : 2022-07-20 , DOI: 10.1016/j.kint.2022.04.040 Johnny Bou Saba 1 , Aravind Cherukuri 2
In this issue, Burton et al. describe a convincing method to identify and enumerate human leukocyte antigen–specific B cells and subsets using biotinylated human leukocyte antigen proteins. Importantly, they demonstrate that these antigen-specific B cells are found at a greater frequency in sensitized kidney transplant recipients when compared with healthy volunteers. Finally, using an indirect antigen-specific enzyme-linked immunosorbent spot assay, they uncover the complexity of B- and T-cell interactions and the influence of regulatory T cells on such interactions in vitro.
中文翻译:
肾移植中的抗原特异性 B 细胞
在这个问题上,伯顿等人。描述了一种使用生物素化的人类白细胞抗原蛋白识别和计数人类白细胞抗原特异性 B 细胞和亚群的令人信服的方法。重要的是,他们证明与健康志愿者相比,这些抗原特异性 B 细胞在致敏肾移植受者中的发现频率更高。最后,使用间接抗原特异性酶联免疫吸附点测定,他们揭示了 B 细胞和 T 细胞相互作用的复杂性以及调节性 T 细胞对体外这种相互作用的影响。