In this study, parylene-C films from plasma deposition as well as thermal deposition were pyrolyzed to prepare a carbon electrode for application in electrochemical immunoassays. Plasma deposition could prepare parylene-C in a faster deposition rate and more precise control over the thickness in comparison with the conventional thermal deposition. To analyze the influence of the deposition method, the crystal and electronic structures of the pyrolyzed parylene-C films obtained via both deposition methods were compared using Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and Raman spectroscopy. For application as a carbon electrode in immunoassays, the electrochemical properties of the pyrolyzed carbon films from two both deposition methods were analyzed, including the double layer capacitance (2.10 μF cm⁻² for plasma deposition and 2.20 μF cm⁻² for thermal deposition), the apparent electron transfer rate (approximately 1.1 × 10⁻³ cm s⁻¹ for both methods), and the electrochemical window (approximately −1.0 ∼ 2.1 V for both methods). Finally, the applicability of the pyrolyzed carbon electrode from parylene-C was demonstrated for the diagnosis of human hepatitis-C using various amperometric methods, such as cyclic voltammetry, chronoamperometry, square-wave voltammetry and differential pulse voltammetry.

中文翻译：

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通过等离子体沉积的聚对二甲苯-C的热解获得用于电化学免疫测定的碳电极

在这项研究中，来自等离子体沉积和热沉积的聚对二甲苯-C 薄膜被热解以制备用于电化学免疫测定的碳电极。与传统的热沉积相比，等离子体沉积可以更快的沉积速率和更精确的厚度控制来制备聚对二甲苯-C。为了分析沉积方法的影响，通过以下方法获得的热解聚对二甲苯-C薄膜的晶体和电子结构使用傅里叶变换红外光谱、X 射线光电子能谱和拉曼光谱对两种沉积方法进行了比较。为了在免疫测定中用作碳电极，分析了两种沉积方法的热解碳膜的电化学性质，包括双层电容（等离子体沉积为2.10 μF cm ^-2和热沉积为2.20 μF cm ^-2 ），表观电子转移率（约 1.1 × 10 ^-3 cm s ^-1两种方法）和电化学窗口（两种方法都约为-1.0~2.1 V）。最后，使用循环伏安法、计时电流法、方波伏安法和差分脉冲伏安法等多种电流分析方法证明了聚对二甲苯-C 的热解碳电极在诊断人类丙型肝炎方面的适用性。