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RsmA3 modulates RpoS through the RetS-Gac-Rsm signalling pathway in response to H2O2 stress in the phytopathogen Pseudomonas syringae
Environmental Microbiology ( IF 5.1 ) Pub Date : 2022-07-15 , DOI: 10.1111/1462-2920.16132
Xun Yu, Cui Meng, Xiaocheng Tan, Yuwei Su, Zhiping Cao, Tzann-Shun Hwang, Lin Li

Reactive oxygen species are a fatal challenge to the plant pathogenic bacterium Pseudomonas syringae. In this study, we reveal that the global regulatory protein RsmA3 from the RetS-Gac/Rsm signalling pathway modulates RpoS in the early-log growth phase in the P. syringae wild-type strain MB03, thereby regulating oxidative tolerance to H2O2 and ultimately affecting pathogenicity to the host plant. Following increased H2O2 by external addition or endogenous induction by menadione, the resistance of the mutant strain ΔretS to H2O2 is significantly enhanced due to rapid increases in the transcription of Rsm-related non-coding small RNAs (nc sRNAs), a sigma factor RpoS, and H2O2-detoxifying enzymes. Moreover, the ΔretS mutant is significantly less pathogenic in cucumber leaves. Seven Rsm-related nc sRNAs (namely, rsmZ, rsmY and rsmX1–5) show functional redundancy in the RetS-Gac-Rsm signalling pathway. External addition of H2O2 stimulates increases in the transcription of both rsmY and rsmZ. Thus, we propose a regulatory model of the RetS-Gac-Rsm signalling pathway in P. syringae MB03 for the regulation of H2O2 tolerance and phytopathogenicity in the host plant.

中文翻译:

RsmA3 通过 RetS-Gac-Rsm 信号通路调节 RpoS 以响应植物病原体丁香假单胞菌中的 H2O2 胁迫

活性氧是对植物病原菌丁香假单胞菌的致命挑战。在这项研究中,我们揭示了来自 RetS-Gac/Rsm 信号通路的全局调节蛋白 RsmA3 在P的早期对数生长期调节 RpoS 。丁香属野生型菌株MB03,从而调节对H 2 O 2的氧化耐受性并最终影响对寄主植物的致病性。通过外部添加或甲萘醌内源诱导增加 H 2 O 2后,突变菌株 Δ retS对 H 2 O 2的抗性由于 Rsm 相关的非编码小 RNA (nc sRNA)、σ 因子 RpoS 和 H 2 O 2 -解毒酶的转录迅速增加,因此显着增强。此外,Δ retS突变体在黄瓜叶中的致病性显着降低。七种与 Rsm 相关的 nc sRNA(即rsmZrsmYrsmX 1-5)在 RetS-Gac-Rsm 信号通路中显示出功能冗余。外部添加 H 2 O 2刺激rsmYrsmZ的转录增加。因此,我们提出了P中 RetS-Gac-Rsm 信号通路的调控模型。. syringae MB03 用于调节宿主植物对 H 2 O 2的耐受性和植物致病性。
更新日期:2022-07-15
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