The colocalization of taurine and zinc transporters (TAUT, ZnTs) has not been explored in retina. Our objective is to evaluate the effect of the intracellular zinc chelator N,N,N,N-tetrakis-(2-pyridylmethyl) ethylenediamine (TPEN) on zinc localization and colocalization TAUT and ZnT-1 (of plasma membrane), 3 (vesicular), and 7 (vesicular and golgi apparatus) in layers of retina by immunohistochemistry. To mark zinc, it was used cell-permeable fluorescent Zinquin ethyl ester. Specific first and secondary antibodies, conjugated with rhodamine or fluorescein-isothiocyanate were used to mark TAUT and ZnTs. The fluorescence results were reported as integrated optical density (IOD). Zinc was detected in all layers of the retina. The treatment with TPEN produced changes in the distribution of zinc in layers of retina less in the outer nuclear layer compared with the control. TAUT was detected in all layers of retina and TPEN chelator produced decrease of IOD in all layers of retina except in the photoreceptor compared with the control. ZnT 1, 3, and 7 were distributed in all retina layers, with more intensity in ganglion cell layer (GCL) and in the layers where there is synaptic connection. For all transporters, the treatment with TPEN produced significant decrease of IOD in layers of retina least in the inner nuclear layer for ZnT1, in the photoreceptor for ZnT3 and in the GCL and outer plexiform layer for ZnT7. The distribution of zinc, TAUT, and ZnTs in the layers of retina is indicative of the interaction of taurine and zinc for the function of the retina and normal operation of said layers.

中文翻译：

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缺锌对大鼠视网膜锌定位、牛磺酸转运和锌转运蛋白的影响

尚未在视网膜中探索牛磺酸和锌转运蛋白（TAUT，ZnTs）的共定位。我们的目标是评估细胞内锌螯合剂N,N,N,N的作用-四-（2-吡啶基甲基）乙二胺（TPEN）通过免疫组织化学对视网膜层中锌定位和共定位 TAUT 和 ZnT-1（质膜）、3（水泡）和 7（水泡和高尔基体）。为了标记锌，使用了细胞可渗透的荧光 Zinquin 乙酯。与罗丹明或异硫氰酸荧光素偶联的特异性一抗和二抗用于标记 TAUT 和 ZnT。荧光结果报告为积分光密度 (IOD)。在视网膜的所有层中都检测到了锌。与对照相比，用 TPEN 治疗产生的锌在视网膜层中分布的变化在外核层中的变化较小。与对照相比，在视网膜的所有层中都检测到了 TAUT，并且 TPEN 螯合剂在视网膜的所有层中产生了 IOD 的降低，除了在感光器中。ZnT 1、3 和 7 分布在所有视网膜层中，在神经节细胞层 (GCL) 和有突触连接的层中强度更高。对于所有转运蛋白，TPEN 处理使视网膜层的 IOD 显着降低，至少在 ZnT1 的内核层、在 ZnT3 的光感受器和 GCL 和 ZnT7 的外丛状层中。视网膜层中锌、TAUT 和 ZnTs 的分布表明牛磺酸和锌对视网膜功能和所述层的正常运作的相互作用。在神经节细胞层 (GCL) 和有突触连接的层中强度更高。对于所有转运蛋白，TPEN 处理使视网膜层的 IOD 显着降低，至少在 ZnT1 的内核层、在 ZnT3 的光感受器和 GCL 和 ZnT7 的外丛状层中。视网膜层中锌、TAUT 和 ZnTs 的分布表明牛磺酸和锌对视网膜功能和所述层的正常运作的相互作用。在神经节细胞层 (GCL) 和有突触连接的层中强度更高。对于所有转运蛋白，TPEN 处理使视网膜层的 IOD 显着降低，至少在 ZnT1 的内核层、在 ZnT3 的光感受器和 GCL 和 ZnT7 的外丛状层中。视网膜层中锌、TAUT 和 ZnTs 的分布表明牛磺酸和锌对视网膜功能和所述层的正常运作的相互作用。