The bioleaching performance in column reactors from a refractory uranium ore by Acidithiobacillus ferrooxidans (A. ferrooxidans), Acidithiobacillus thiooxidans (A. thiooxidans) and Leptospirillum ferriphilum (L. ferriphilum) consortium at different depth was assessed. It showed an obvious depth-induced deviation for uranium bioleaching in the column reactors. At the 0–63 cm depth in a 100 cm column reactor, the uranium leaching concentration and peak uranium concentrations were significantly and positively correlated with depth, and conversely decreased when the depth increased further, a peak delay occurred. Furthermore, the residue characteristics were detected by SEM and EDS mapping. The results indicated that a dense passivation layer was formed at 42–63 cm, which seriously hindered the penetration of ions. Additionally, the bacterial community analysis showed that in the early-bioleaching phase, a better balance between iron-oxidizing and sulfur-oxidizing bacteria could be maintained (close to 1:1) in the solution in the upper layer of the column reactor (about 21 cm) for more efficient ore decomposition in the column reactor. In the late-bioleaching phase, the content of sulfur-oxidizing bacteria exceeds twice that of iron-oxidizing bacteria especially in the lower layer, which was not favorable for the leaching of uranium. Lastly, a mechanism model for depth-induced deviation of column bioleaching for uranium bioleaching was established coupling with community succession.

氧化亚铁硫杆菌( A. ferrooxidans ) , 氧化硫硫杆菌( A. thiooxidans ) 和嗜铁钩端螺旋体( L. ferriphilum )对难处理铀矿石在柱反应器中的生物浸出性能) 对不同深度的财团进行了评估。它显示了塔式反应器中铀生物浸出的明显深度引起的偏差。在 100 cm 柱式反应器中 0~63 cm 深度处，铀浸出浓度和峰值铀浓度与深度呈显着正相关，反之随着深度增加而降低，出现峰值延迟。此外，通过 SEM 和 EDS 映射检测残留物特征。结果表明，在42-63 cm处形成了致密的钝化层，严重阻碍了离子的穿透。此外，细菌群落分析表明，在早期生物浸出阶段，铁氧化细菌和硫氧化细菌之间可以保持更好的平衡（接近 1：1）在塔式反应器上层（约21厘米）的溶液中，用于在塔式反应器中更有效地分解矿石。在生物浸出后期，硫氧化菌的含量超过铁氧化菌的两倍，尤其是在下层，不利于铀的浸出。最后，建立了与群落演替耦合的铀生物浸出柱浸出深度偏差的机理模型。