In this work, biphenyl was used as carbon source to enrich microorganisms from polychlorinated biphenyls (PCBs)-contaminated paddy soil samples, and the taxonomic structures in both of the soil samples and the fourth-generation enrichments were examined with high-throughput sequencing. Single cells were isolated from the enrichments via single cell sorting technology named Laser Induced Visualized Ejection Separation Technology (LIVEST) and also traditional single colony sorting, and the genera of the isolates were identified using 16S rRNA sequencing. The results from high-throughput sequencing present that enrichment from generation to generation can considerably change the microbial community. Comparing the two sorting methods, the LIVEST is more time-saving and cell-targeted for microbial resource exploration. Based on the further verification of biphenyl degradation, it was found that some strains belonging to genera Macrococcus, Aerococcus and Metabacillus are capable in degrading biphenyl, which have not been reported yet.

在这项工作中，使用联苯作为碳源从多氯联苯 (PCBs) 污染的稻田土壤样品中富集微生物，并通过高通量测序检查土壤样品和第四代富集物的分类结构。通过称为激光诱导可视化喷射分离技术 (LIVEST) 的单细胞分选技术和传统的单菌落分选技术从富集物中分离出单细胞，并使用 16S rRNA 测序鉴定分离物的属。高通量测序的结果表明，一代又一代的富集可以显着改变微生物群落。对比两种分选方式，LIVEST更省时，更针对细胞进行微生物资源探索。大球菌、气球菌和变形杆菌具有降解联苯的能力，尚未见报道。