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An enzyme-particle hybrid ink for one step screen-printing and long-term metabolism monitoring
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2022-07-15 , DOI: 10.1016/j.aca.2022.340168
Chengcheng Wang 1 , Xinran Zhang 2 , Yuqiao Liu 2 , Junmin Li 2 , Ling Zhu 2 , Yan Lu 3 , Xishan Guo 4 , Dajing Chen 2
Affiliation  

Targeting the long-term monitoring of biological carbohydrate metabolism, we developed a one-step screen-printing method to fabricate electrochemical sensors using an enzyme microparticle hybrid ink. Most enzymes have low stability in high temperatures and organic solvents, making conventional enzyme modification a bottom-up procedure to be performed after electrode fabrication, resulting in inactivation and detachment in long-term work. Enzyme-loaded microparticles prepared by manganese carbonate co-precipitation had higher stability than free enzymes, which could to be mixed directly with carbon paste for direct screen-printing. Due to the co-printing immobilization and the local hydration environment in enzyme particles, the prepared electrodes exhibited higher long-term operational stability than the conventional multi-step cross-linking method. In the sensing applications, we prepared microparticles loaded with single enzyme (glucose oxidase) and dual enzymes (β-galactosidase and glucose oxidase) for glucose and lactose monitoring, respectively. Both electrodes can accurately measure the consumption of the corresponding carbohydrates throughout the cell or bacterial culture period thus providing a sensing platform for bio-metabolic monitoring and drug screening.



中文翻译:

用于一步丝网印刷和长期代谢监测的酶-颗粒混合墨水

针对生物碳水化合物代谢的长期监测,我们开发了一种使用酶微粒混合墨水制造电化学传感器的一步丝网印刷方法。大多数酶在高温和有机溶剂中的稳定性较低,使得常规的酶修饰成为电极制造后自下而上的过程,导致在长期工作中失活和脱离。碳酸锰共沉淀法制备的载酶微粒比游离酶具有更高的稳定性,可直接与碳糊混合进行直接丝网印刷。由于酶颗粒中的共印固定和局部水合环境,与传统的多步交联方法相比,制备的电极表现出更高的长期操作稳定性。在传感应用中,我们制备了载有单酶(葡萄糖氧化酶)和双酶(β-半乳糖苷酶和葡萄糖氧化酶)的微粒,分别用于监测葡萄糖和乳糖。两个电极都可以准确测量整个细胞或细菌培养期间相应碳水化合物的消耗量,从而为生物代谢监测和药物筛选提供传感平台。

更新日期:2022-07-19
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